Bansi Dhar Malhotra

Learn More
Nanostructured cerium oxide (NanoCeO(2)) film deposited onto indium-tin-oxide (ITO) glass substrate by solution casting has been used for immobilization of horseradish peroxidase (HRP) via physiosorption technique. X-ray diffraction (XRD), scanning electron microscopy (SEM), UV-vis and electrochemical techniques have been utilized for characterization of(More)
Direct electrode transfer between enzyme and the electrode in biosensors requires high efficiency therefore, synthetic replacement for oxygen led to the development of enzyme mediators and modified electrodes in biosensor fabrication. In this context, a number of electron acceptors and complexes have been used. Present paper gives an overview of various(More)
A 20-mer thiolated oligonucleotide probe (th-ssDNA) specific to Neisseria gonorrhoeae immobilized onto a sol-gel derived nano-structured zinc oxide (ZnO) film dip-coated onto an indium-tin-oxide (ITO) glass substrate has been used for the fabrication of a DNA biosensor for sexually transmitted disease (gonorrhoea) detection using hybridization technique.(More)
Double stranded calf thymus deoxyribonucleic acid entrapped polypyrrole-polyvinyl sulphonate (dsCT-DNA-PPy-PVS) films fabricated onto indium-tin-oxide (ITO) coated glass plates have been used to detect organophosphates such as chlorpyrifos and malathion. These disposable dsCT-DNA-PPy-PVS/ITO bioelectrodes have been characterized using cyclic voltammetry,(More)
Recently, conducting polymers have attracted much interest in the development of biosensors. The electrically conducting polymers are known to possess numerous features, which allow them to act as excellent materials for immobilization of biomolecules and rapid electron transfer for the fabrication of efficient biosensors. In the present review an attempt(More)
Cholesterol oxidase (ChOx) has been covalently immobilized onto electrophoretically deposited conducting polymer film (on indium-tin-oxide (ITO) glass plate) derived from nano-structured polyaniline (PANI) colloidal suspension using N-ethyl-N'-(3-dimethylaminopropyl) corbodiimide (EDC) and N-hydroxysuccinimide (NHS) chemistry. These PANI/ITO and(More)
Biosensors have recently gained much attention in the field of health care for the management of various important analytes in a biological system. The area achieved tremendous progress from the time when the first Clark electrode for measurement of glucose was realized. Advances in the biosensor design are appearing at a high rate as these devices play(More)
Nanocomposite film composed of polyaniline (PANI) and multiwalled carbon nanotubes (MWCNT), prepared electrophoretically onto indium tin oxide (ITO)-coated glass plate, was used for covalent immobilization of cholesterol oxidase (ChOx) via N-ethyl-N'-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) chemistry. Results of linear sweep(More)
Avidin-modified polyaniline (PANI) electrochemically deposited onto a Pt disk electrode has been utilized for direct detection of Escherichia coli by immobilizing a 5'-biotin-labeled E. coli probe (BdE) using a differential pulse voltammetric technique in the presence of methylene blue as a DNA hybridization indicator. Depending on the target sample and the(More)
Applications of conducting polymers to biosensors have recently aroused much interest. This is because these molecular electronic materials offer control of different parameters such as polymer layer thickness, electrical properties and bio-reagent loading, etc. Moreover, conducting polymer based biosensors are likely to cater to the pressing requirements(More)