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Journals and Conferences
A catabolite derepressed mutant of a glucoamylolytic strain ofAspergillus terreus 4 produced 1.8 times more glucoamylase than its parent; the crude enzyme preparation appreciably degraded raw starchy materials like wheat, rice, potato and barley.
EtBr binding to DNA has been used to determine the superhelical density of Goat mtDNA. The differential binding of closed circular and nicked circular DNA is estimated by simple fluorescence measurements, which is a function of state of DNA supercoiling. The method is simple and quick over the cesium chloride density gradient centrifugation studies.
Southern blot hybridization analysis of genomic DNAs digested with restriction endonuclease EcoR I and Ava II from Mus musculus domesticus, Mus booduga and Mus terricolor with a cloned repetitive DNA fragment of Mus booduga as a probe showed difference in restriction pattern of this DNA in these three species. Further Southern analysis of the BamH I… (More)
A simple rapid method for isolation of genomic DNA from cervical scrape cells has been standardized. Sufficient amount of good quality genomic DNA was isolated from each cervical scrape specimen. Total 120 cervical scrape specimens from wide spectrum of cervical lesions were analysed for the presence of HPV 16 DNA by Southern hybridization using 32P… (More)
Amplification of circular and linear mitochondrial DNAs (mtDNA) has been checked by PCR using two thermostable polymerases. The results demonstrated that while Taq polymerase could amplify both circular and linear mtDNAs, the Vent polymerase can only amplify linear DNA.