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A low voltage, two-level-metal, and multi-layer insulator electrowetting-on-dielectric (EWD) platform is presented. Dispensing 300pl droplets from 140nl closed on-chip reservoirs was accomplished with as little as 11.4V solely through EWD forces, and the actuation threshold voltage was 7.2V with a 1Hz voltage switching rate between electrodes. EWD devices(More)
Advances in digital microfluidics and integrated sensing hold promise for a new generation of droplet-based biochips that can perform multiplexed assays to determine the identity of target molecules. Despite these benefits, defects and erroneous fluidic operations remain a major barrier to the adoption and deployment of these devices. We describe the first(More)
The high mortality rate of sepsis has been attributed to delayed pathogen identification. Hence, a fluidic device is being developed to accelerate the procedures from DNA purification to qPCR sequence detection. Demonstrated herein is the key feature the device: the use of EWD droplet actuation to manipulate the eluent generated by an immiscible phase(More)
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