Balamurali Kannan

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In this study, a paper-based point-of-care (POC) colorimetric biosensor was developed for the detection of lactate dehydrogenase in serum using a nonporous, oxygen impermeable reversibly gelling polysaccharide material based on pullulan. The pullulan could be printed onto paper surfaces along with all required assay reagents, providing a means for(More)
We report on a paper device capable of carrying out target-induced rolling circle amplification (RCA) to produce massive DNA amplicons that can be easily visualized. Interestingly, we observed that RCA was more proficient on paper than in solution, which we attribute to a significantly higher localized concentration of immobilized DNA. Furthermore, we have(More)
A simple and inexpensive method is reported for the long-term stabilization of enzymes and other unstable reagents in premeasured quantities in water-soluble tablets (cast, not compressed) made with pullulan, a nonionic polysaccharide that forms an oxygen impermeable solid upon drying. The pullulan tablets dissolve in aqueous solutions in seconds, thereby(More)
A soft computing model for nowcasting of Yes/No rain situations, with a lead time of 2 h, is developed over DWR station at Satish Dhawan Space Centre (SDSD), Shriharikota (13.66°N, 80.23°E) using Doppler weather radar (DWR) reflectivity imageries. Primarily, precipitating systems of mesoscale, i.e. meso-gamma (2-20 km), meso-beta (20-200 km) and meso-alpha(More)
A study is carried out to investigate the sensitivity of Z-R relations and spatial variability of error in a Doppler Weather Radar (DWR) measured rain intensity. For this purpose, observations from a DWR at Satish Dhawan Space Centre, SHAR (13.66°N, 80.23°E) and five units of automatic tipping bucket rain gauges around the DWR are utilized. It is found that(More)
A simple, elegant method for the formation of a continuous stationary phase gradient for use in chromatographic separations is described. Its applicability to separation science is demonstrated using thin-layer chromatography as a test case. Gradient stationary phases were formed on activated High Performance Thin-Layer Chromatography (HP-TLC) plates using(More)
The reactivity of a series of substituted aminoalkoxysilanes for surface amine gradient formation has been studied using a newly developed time-based exposure method termed controlled-rate infusion (CRI). The aminoalkoxysilanes used include those that contain primary, secondary, and tertiary monoamines as well as more than one amine group (diamine and(More)
Surface charge gradients have been formed on the inside surface of 75 μm i.d. silica capillaries via controlled rate infusion using 3-aminopropyltriethoxysilane as the reactive precursor. These 400 mm length gradients have been characterized using spatially resolved streaming potential measurements, from which the zeta potential as a function of distance(More)
We describe a versatile and simple method to perform sequential reactions on paper analytical devices by stacking dry pullulan films on paper, where each film contains one or more reagents or acts as a delay layer. Exposing the films to an aqueous solution of the analyte leads to sequential dissolution of the films in a temporally controlled manner followed(More)
The utility of hydrophobic wax barriers in paper-based lateral flow and multiwell devices for containment of aqueous solvents was extended to organic solvents and challenging aqueous surfactant solutions by preparation of a three layer barrier, consisting of internal pullulan impregnated paper barriers surrounded by external wax barriers. When paper(More)