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Use of the cell wall precursor lipid II by a pore-forming peptide antibiotic.
TLDR
It is shown that vancomycin and the antibacterial peptide nisin Z use the same target: the membrane-anchored cell wall precursor Lipid II, thus causing the peptide to be highly active (in the nanomolar range). Expand
Specific Binding of Nisin to the Peptidoglycan Precursor Lipid II Combines Pore Formation and Inhibition of Cell Wall Biosynthesis for Potent Antibiotic Activity*
TLDR
Genetically engineered nisin variants are used to identify the structural requirements for the interaction of the peptide with lipid II, and the remaining in vivo activity is found to result from the unaltered capacity of the mutated peptide to bind to lipid II and thus to inhibit its incorporation into the peptidoglycan network. Expand
Identification of FtsW as a transporter of lipid-linked cell wall precursors across the membrane
TLDR
This study provides the first biochemical evidence for the involvement of an essential protein in the transport of lipid‐linked cell wall precursors across biogenic membranes. Expand
Visualizing detergent resistant domains in model membranes with atomic force microscopy
TLDR
Atomic force microscopy is used to study supported lipid bilayers, consisting of a fluid phosphatidylcholine, sphingomyelin and cholesterol, and the presence of cholesterol was found to induce bilayer coupling. Expand
Molecular basis of glycoalkaloid induced membrane disruption.
TLDR
A molecular model for glycoalkaloid induced membrane disruption is presented and the importance of sugar-sugar interactions was illustrated by the high synergistic effect between alpha-chaconine and alpha-solanine, the leakage enhancing effect of glycolipids, and the almost complete loss of activity after deleting one or more mono-saccharides from the glycoalksaloids. Expand
The C Terminus of SecA Is Involved in Both Lipid Binding and SecB Binding (*)
TLDR
The observation that the SecA mutant protein lacking the C-terminal 70 residues had a strongly reduced ability to mediate binding of SecB-precursor complexes to inverted inner membrane vesicles demonstrates that the C terminus of SecA is also involved in SecB binding. Expand
Membrane damage by human islet amyloid polypeptide through fibril growth at the membrane
TLDR
A hypothesis that growth of hIAPP fibrils at the membrane causes membrane damage is proposed, which provides an additional mechanism next to the previously proposed role of oligomers as the main cytotoxic species of amyloidogenic proteins. Expand
An Electrostatic/Hydrogen Bond Switch as the Basis for the Specific Interaction of Phosphatidic Acid with Proteins*
TLDR
It is demonstrated that this electrostatic/hydrogen bond switch turns the phosphate of PA into an effective and preferred docking site for lysine and arginine residues, and PA may well be nature's preferred membrane lipid for interfacial insertion of positively charged membrane protein domains. Expand
Lipid II Is an Intrinsic Component of the Pore Induced by Nisin in Bacterial Membranes*
TLDR
It is shown here that Lipid II is not only the receptor for nisin but an intrinsic component of the pore formed by nisin, and a new model for the pORE complex that includes Lipid I and variants thereof is presented. Expand
Natamycin Blocks Fungal Growth by Binding Specifically to Ergosterol without Permeabilizing the Membrane*
TLDR
It is demonstrated that natamycin acts via a novel mode of action and blocks fungal growth by binding specifically to ergosterol in model membranes. Expand
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