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Expression of dha Operon Required for 1,3-PD Formation in Escherichia coli and Saccharomyces cerevisiae
TLDR
The results demonstrated that the recombinant S. cerevisiae strain W303-1A-BT that can produce 1,3-PD at low cost was constructed and might open a novel way to a safe and cost-efficient method for microbial production of 1, 3-PD. Expand
Construction of a Novel Expression System in Klebsiella pneumoniae and its Application for 1,3-Propanediol Production
TLDR
The first reported expression system of Klebsiella pneumoniae has paved the way for improvement of 1,3-PD production and will be available and efficient for other heterologous gene expression in K. pneumoniae. Expand
Construction of a novel recombinant Escherichia coli strain capable of producing 1,3–propanediol and optimization of fermentation parameters by statistical design
TLDR
A mathematical model was developed and compared with the values obtained by other runs in the experimental design, the optimized medium resulted in a significant increase in the yield of 1,3-propanediol, which makes the engineered strain have potential application in the conversion of glycerol to 1, 3-pro panediol on an industrial scale. Expand
Expression of a Bacillus subtilis pectate lyase gene in Pichia pastoris
TLDR
The cloning and functional expression of the bacterial Bacillus subtilis gene encoding alkaline pectate lyase in P. pastoris is reported for the first time. Expand
Improved 1, 2, 4-butanetriol production from an engineered Escherichia coli by co-expression of different chaperone proteins
TLDR
The potential application of chaperone proteins in microorganism engineering to get high production of target compounds as an effective and valuable tool is shown. Expand
budC knockout in Klebsiella pneumoniae for bioconversion from glycerol to 1,3‐propanediol
TLDR
The results of the mutant fed‐batch fermentation showed that the 1,3‐PDO concentration, productivity per cell dry weight, and conversion rate showed increasing by 10%, 15%, and 11% compared with the parent strain, which implies that K. pneumoniae possesses a pathway of the 2, 3‐BD cycle as a replenishment pathway. Expand
Production of 1,3-propanediol from glycerol by engineered Escherichia coli using a novel co- expression vector
TLDR
1,3-Propanediol has versatile applications in polymers, cosmetics, foods and medicines and the co-expression system, T7RNA polymerase promoter helps to consolidate the functions of glycerol dehydratase genedhaB and 1, 3-propanediol oxidoreductase gene dhaT. Expand
Ethanol fermentation from non-detoxified lignocellulose hydrolysate by a multi-stress tolerant yeast Candida glycerinogenes mutant.
TLDR
C. glycerinogenes UG-21 with high tolerance to elevated temperature, acetic acid, and furfural was obtained and applied in lignocellulose-based ethanol production and exhibited highly-efficient degradation ability tofurfural. Expand
Efficient secretory expression of an alkaline pectate lyase gene from Bacillus subtilis in E. coli and the purification and characterization of the protein
TLDR
Analysis of polygalacturonic acid (PGA) degradation products by electrospray ionization (ESI)-mass spectrometry (MS) indicated that PL produced a mixture of unsaturated oligo-galacturonides including unsaturated tri-galactoronic acid and unsaturated bi-galactic acid but not unsaturated mono-galactsonic acid. Expand
Itaconic acid production in microorganisms
TLDR
Two types of pathway for itaconic acid biosynthesis as well as recent advances by metabolic engineering strategies and process optimization are reviewed to enhance itaconed acid productivity in native producers and heterologous hosts. Expand
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