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Ellman's reagent: 5,5'-dithiobis(2-nitrobenzoic acid)--a reexamination.
The molar absorption coefficient of the dianion of TNB is 14,150 at 412 nm in dilute aqueous salt solutions and this value was confirmed independently by reduction of purified DTNB with cysteine. Expand
Reassessment of Ellman's reagent.
Recent advances in the chemistry of an old enzyme, urease
Urease was the first enzyme to be crystallized and shown to be a protein. Some 50 years after its crystallization, it was shown to be the first example of the nickel metalloenzymes. Whereas urea isExpand
Urea and urease.
Etude comparee de la degradation enzymatique et non enzymatsique de l'uree et son mecanisme de reaction. Expand
Jack bean urease: the first nickel enzyme
The article defines the development of our present understanding of the urease molecule. It describes physical and chemical properties of the enzyme, the interaction of inhibitors with the activeExpand
Properties of a purple phosphatase from red kidney bean: a zinc-iron metalloenzyme
A purple phosphatase from the red kidney bean Phaseolus vulgaris has been purified to homogeneity and characterized. It resembles sweet potato purple acid phosphatase in being a dimer of approx. 130Expand
Jack bean urease: the first nickel enzyme
Jack bean urease is the first example of a nickel metalloenzyme [1–3]. Reassessment of the molecular properties of the enzyme shows that the native enzyme has a molecular weight (Mr) of 590.000±Expand
Jack bean urease (EC V. On the mechanism of action of urease on urea, formamide, acetamide, N-methylurea, and related compounds.
A mechanism of action for urease is proposed which involves initially an O-bonded complex between urea and an active-site Ni2+ ion and subsequently anO-bonding carbamato-enzyme intermediate. Expand