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Demonstration of CRISPR/Cas9/sgRNA-mediated targeted gene modification in Arabidopsis, tobacco, sorghum and rice
The type II CRISPR/Cas system from Streptococcus pyogenes and its simplified derivative, the Cas9/single guide RNA (sgRNA) system, have emerged as potent new tools for targeted gene knockout inExpand
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Identification of cross-linked peptides from complex samples
We have developed pLink, software for data analysis of cross-linked proteins coupled with mass-spectrometry analysis. pLink reliably estimates false discovery rate in cross-link identification and isExpand
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TAL nucleases (TALNs): hybrid proteins composed of TAL effectors and FokI DNA-cleavage domain
We report the creation and initial characterization of a group of rare-cutting, site-specific DNA nucleases produced by fusion of the restriction enzyme FokI endonuclease domain with the high-specificity DNA-binding domains of AvrXa7 and PthXo1. Expand
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Modularly assembled designer TAL effector nucleases for targeted gene knockout and gene replacement in eukaryotes
Recent studies indicate that the DNA recognition domain of transcription activator-like (TAL) effectors can be combined with the nuclease domain of FokI restriction enzyme to produce TAL effectorExpand
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Risk assessment and sources of polycyclic aromatic hydrocarbons in agricultural soils of Huanghuai plain, China.
  • B. Yang, N. Xue, +6 authors Bo Liu
  • Environmental Science, Medicine
  • Ecotoxicology and environmental safety
  • 1 October 2012
The profiles and sources of polycyclic aromatic hydrocarbons (PAHs) were analyzed in 227 agricultural surface soils from the Huanghuai plain, China. The total concentrations of 16 PAHs varied fromExpand
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Code-Assisted Discovery of TAL Effector Targets in Bacterial Leaf Streak of Rice Reveals Contrast with Bacterial Blight and a Novel Susceptibility Gene
Bacterial leaf streak of rice, caused by Xanthomonas oryzae pv. oryzicola (Xoc) is an increasingly important yield constraint in this staple crop. A mesophyll colonizer, Xoc differs from X. oryzaeExpand
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Source apportionment of polycyclic aromatic hydrocarbons in soils of Huanghuai Plain, China: comparison of three receptor models.
Receptor models are useful tools to identify sources of a specific pollutant and to estimate the quantitative contributions of each source based on environmental data. This paper reports onExpand
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An Agrobacterium‐delivered CRISPR/Cas9 system for high‐frequency targeted mutagenesis in maize
Summary CRISPR/Cas9 is a powerful genome editing tool in many organisms, including a number of monocots and dicots. Although the design and application of CRISPR/Cas9 is simpler compared to otherExpand
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Methods to measure the lateral diffusion of membrane lipids and proteins.
In this chapter, we discuss methods to measure lateral mobility of membrane lipids and proteins using techniques based on the light microscope. These methods typically sample lateral mobility in veryExpand
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pNovo: de novo peptide sequencing and identification using HCD spectra.
De novo peptide sequencing has improved remarkably in the past decade as a result of better instruments and computational algorithms. However, de novo sequencing can correctly interpret onlyExpand
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