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Formation of Polyesters by Pseudomonas oleovorans: Effect of Substrates on Formation and Composition of Poly-(R)-3-Hydroxyalkanoates and Poly-(R)-3-Hydroxyalkenoates.
It is shown that the intermediate 3-Hydroxy fatty acids can also be polymerized to intracellular poly-(R)-3-hydroxyalkanoates (PHAs) when the medium contains limiting amounts of essential elements, such as nitrogen. Expand
Occurrence, synthesis and medical application of bacterial polyhydroxyalkanoate.
It was found that PHA producing bacteria are able to grow simultaneously limited by carbon and nitrogen substrates, and it became possible to produce PHA at high yields on toxic substrate and also control its composition accurately (tailor-made synthesis). Expand
Degradation of chlorinated aliphatic hydrocarbons by Methylosinus trichosporium OB3b expressing soluble methane monooxygenase.
Degradation of trichloroethylene (TCE) by the methanotrophic bacterium Methylosinus trichosporium OB3b was studied by using cells grown in continuous culture. TCE degradation was a strictlyExpand
Gene Cloning and Characterization of Multiple Alkane Hydroxylase Systems in Rhodococcus Strains Q15 and NRRL B-16531
The presence of multiple alkane hydroxylases in the two rhodococcal strains is reminiscent of other multiple-degradative-enzyme systems reported in Rhodococcus. Expand
Functional analysis of alkane hydroxylases from gram-negative and gram-positive bacteria.
Three recombinant host strains are developed for the functional analysis of the novel alkane hydroxylase genes and sequence comparisons show that the level of protein sequence identity between the homologs is as low as 35%, and that the Pseudomonas alkane Hydroxylases are as distantly related to each other as to the remaining alkanes. Expand
Diversity of Alkane Hydroxylase Systems in the Environment
The first step in the aerobic degradation of alkanes by bacteria, yeasts, and fungi is catalyzed by oxygenases. These enzymes, which introduce oxygen atoms derived from molecular oxygen into theExpand
Methods for Intense Aeration, Growth, Storage, and Replication of Bacterial Strains in Microtiter Plates
The optimization of oxygen transfer rates in deep-well microtiter plates and the development of a replication system allowing the simultaneous and reproducible sampling of 96 frozen glycerol stock cultures while the remaining culture volume remains frozen are reported on. Expand
Molecular screening for alkane hydroxylase genes in Gram-negative and Gram-positive strains.
Highly degenerate oligonucleotides for polymerase chain reaction (PCR) amplification of genes related to the Pseudomonas oleovorans GPo1 and Acinetobacter sp. Expand
An Alkane-Responsive Expression System for the Production of Fine Chemicals
The alkane-responsive regulatory system of the OCT plasmid-located alk genes of Pseudomonas oleovorans GPo1 was utilized, and the N-terminal amino acid sequence of an AlkS-LacZ fusion protein was found to be consistent with the predicted DNA sequence. Expand
Towards a Biocatalyst for (S)-Styrene Oxide Production: Characterization of the Styrene Degradation Pathway of Pseudomonas sp. Strain VLB120
To assess the usefulness of the enzymes for the production of enantiomerically pure styrene oxide, the enantiospecificities of the reactions involved were investigated and confirmed a probable function as a phenylacetaldehyde dehydrogenase. Expand