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Distinct binding sites of Ala48-hirudin1-47 and Ala48-hirudin48-65 on alpha-thrombin.
The interaction of alpha-thrombin with Ala48-hirudin, Ala48-hirudin1-47, and Ala48-hirudin48-65 was analyzed. Mutations at Pro48 were found to cause only slight changes in the kon (human: 3.1 +/- 0.3Expand
Effects of zinc chloride on glutathione and glutathione synthesis rates in various lung cell lines
Effect of zinc on cellular GSH content, glutathione reductase (GR) activity, and GSH synthesis were investigated and it is assumed the inhibition of GR activity and the associated increase of GSSG could possibly represent a main zinc-mediated toxic cellular effect. Expand
Zinc toxicity in various lung cell lines is mediated by glutathione and GSSG reductase activity
It is concluded that reduction of GSSG is reduced in zinc-exposed cells, and protection of GSH oxidation by various antioxidants as well as enhancement of G SH content are expected to be mechanisms of diminishing toxic cellular effects after exposure to zinc. Expand
Myoglobin with modified tetrapyrrole chromophores: binding specificity and photochemistry.
Complexes were prepared of horse heart myoglobin with derivatives of (bacterio)chlorophylls and the linear tetrapyrrole, phycocyanobilin andStructural factors important for binding are the presence of a central metal with open ligation site, and the absence of the hydrophobic esterifying alcohol, phytol. Expand
Photostability of Bacteriochlorophyll a and Derivatives: Potential Sensitizers for Photodynamic Tumor Therapy
The spectral signature of the major component of these products was characteristic of linear open-chain tetrapyrroles, but they lacked the characteristic protonation–deprotonation behavior and reactivity of bilins with Zn++. Expand
The role of aromatic phenylalanine residues in binding carotenoid to light-harvesting model and wild-type complexes.
Binding and specific functionalisation of Crt in the model and WT light-harvesting complex is reliant on the aromatic residue phenylalanine, and the use of the light- Harvesting complex as a model system substantiates the notion that this residue is a key factor for the binding of carotenoid Crt to transmembrane proteins. Expand
Distribution of Chlorophyll‐ and Bacteriochlorophyll‐derived Photosensitizers in Human Blood Plasma
The distribution of 21 (bacterio)chlorophyll derivatives among human blood plasma fractions was studied by iodixanol gradient ultracentrifugation and in situ absorption spectroscopy and influenced by application in Cremophor® EL. Expand
Effect of zinc chloride on GSH synthesis rates in various lung cell lines.
No compensating increase in GSH synthesis rates was found after zinc-mediated cellular GSH depletion, and in all cell lines the availability of Cys was not affected by exposure to zinc. Expand
Photochemistry of Bacteriochlorophylls in Human Blood Plasma: 1. Pigment Stability and Light‐induced Modifications of Lipoproteins
The stability of all pigments is increased in blood plasma, compared with monomeric solutions; Pd‐bacteriopheophorbide is much more stable than the other two derivatives; and there is furthermore evidence for photobleaching under anoxic conditions. Expand
Photochemistry of Bacteriochlorophylls in Human Blood Plasma: 2. Reaction Mechanism Investigated by Product Analysis and Deuterium Isotope Effect
A mechanistic switch is concluded in the mechanism of photo‐oxidation in two lipoprotein fractions derived from human blood plasma that preferentially accumulate these pigments, and a possible mechanism is discussed. Expand