One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products.
- K. Datsenko, B. Wanner
- Biology, EngineeringProceedings of the National Academy of Sciences…
- 6 June 2000
A simple and highly efficient method to disrupt chromosomal genes in Escherichia coli in which PCR primers provide the homology to the targeted gene(s), which should be widely useful, especially in genome analysis of E. coli and other bacteria.
Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection
These mutants—the ‘Keio collection’—provide a new resource not only for systematic analyses of unknown gene functions and gene regulatory networks but also for genome‐wide testing of mutational effects in a common strain background, E. coli K‐12 BW25113.
Conditional-Replication, Integration, Excision, and Retrieval Plasmid-Host Systems for Gene Structure-Function Studies of Bacteria
- A. Haldimann, B. Wanner
- BiologyJournal of Bacteriology
- 1 November 2001
A series of powerful and versatile conditional-replication, integration, and modular (CRIM) plasmids that encode different forms of resistance and can be used together in the same cell for stable expression of complex metabolic or regulatory pathways from diverse sources are developed.
Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110
- K. Hayashi, N. Morooka, T. Horiuchi
- BiologyMolecular Systems Biology
- 21 February 2006
Direct DNA sequencing of PCR products for the 251 regions with short indel and base disparities revealed that only eight sites are true differences, and errors in the original MG1655 sequence were mostly within portions sequenced with out‐dated technology based on radioactive chemistry.
Molecular memory of prior infections activates the CRISPR/Cas adaptive bacterial immunity system.
- K. Datsenko, K. Pougach, A. Tikhonov, B. Wanner, K. Severinov, E. Semenova
- BiologyNature Communications
- 10 July 2012
It is shown that matching sequences that are no longer able to elicit defense, still guide the CRISPR/Cas acquisition machinery to foreign DNA, thus making the spacer acquisition process adaptive and leading to restoration of CRISpr/Cas-mediated protection.
Interference by clustered regularly interspaced short palindromic repeat (CRISPR) RNA is governed by a seed sequence
- E. Semenova, M. M. Jore, K. Severinov
- BiologyProceedings of the National Academy of Sciences
- 6 June 2011
It is proposed that the crRNA seed sequence plays a role in the initial scanning of invader DNA for a match, before base pairing of the full-length spacer occurs, which may enhance the protospacer locating efficiency of the E. coli Cascade complex.
Transcriptome analysis of all two‐component regulatory system mutants of Escherichia coli K‐12
Results support the view that a network of functional interactions, such as cross‐regulation, exists between different two‐component systems of Escherichia coli.
The phosphate regulon and bacterial virulence: a regulatory network connecting phosphate homeostasis and pathogenesis.
- M. Lamarche, B. Wanner, S. Crépin, J. Harel
- BiologyFEMS Microbiology Reviews
- 1 May 2008
The Pho regulon is clearly not a simple regulatory circuit for controlling phosphate homeostasis; it is part of a complex network important for both bacterial virulence and stress response.
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