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Compilation and analysis of sigma(54)-dependent promoter sequences.
TLDR
The use of the extended consensus increases the probability of identifying genuine -24/-12 promoters and the effect of several reported mutations on RNA-polymerase binding and promoter strength is discussed in the light of the updated consensus. Expand
Compilation and analysis of σ54-dependent promoter sequences
TLDR
The use of the extended consensus increases the probability of identifying genuine -24/-12 promoters and the effect of several reported mutations on RNA-polymerase binding and promoter strength is discussed in the light of the updated consensus. Expand
Suicide inactivation of peroxidases and the challenge of engineering more robust enzymes.
TLDR
The catalytic cycle of peroxidases and the mechanism of the suicide inactivation process are discussed to establish a broad knowledge base for future rational protein engineering. Expand
Mechanism of Pseudomonas aeruginosa RhlR Transcriptional Regulation of the rhlAB Promoter
TLDR
It is shown that RhlR binds to a specific sequence in the rhlAB regulatory region, both in the presence and in the absence of its autoinducer, and data suggest that in the former case it activates transcription, whereas in the latter it acts as a transcriptional repressor of this promoter. Expand
Evolutionary and structural diversity of fungal laccases
TLDR
The topology of the phylogenetic trees indicate that a single monophyletic branch exists for fungal laccases and that laccase isozyme genes may have evolved independently, possibly through duplication-divergence events. Expand
Alkali and halide-resistant catalysis by the multipotent oxidase from Marinomonas mediterranea.
TLDR
It is reported that the multipotent polyphenol oxidase PPO1 from the marine bacterium Marinomonas mediterranea remained functional above neutral pH presenting high specificity for phenolic compounds, in particular for methoxyl-substituted mono-phenols and catechols. Expand
Phylogenetic and biochemical characterisation of a recombinant laccase from Trametes versicolor.
TLDR
The cloning and characterisation of a new variant of laccase from T. versicolor and its expression in Saccharomyces cerevisiae is described and a proposal for a new nomenclature of this fungus laccases according to their phylogenetic relationships is proposed. Expand
Microsomal Transformation of Organophosphorus Pesticides by White Rot Fungi
TLDR
Results suggest the involvement of a cytochrome P450 in the transformation of organophosphorus pesticides by differentwhite-rot fungi strains, supported by specific inhibition experiments and the stringent requirement for NADPH during the in vitroassays. Expand
Two mammalian glucosamine‐6‐phosphate deaminases: a structural and genetic study
TLDR
Interestingly the region with the largest differences, when compared with the Escherichia coli homologue, was found to be close to the active site and can be related to the kinetic and allosteric properties of both mammalian enzymes. Expand
Rhizobium etli Genetically Engineered for the Heterologous Expression of Vitreoscilla sp. Hemoglobin: Effects on Free-Living and Symbiosis
TLDR
The expression of vhb in free-living R. etli grown under most limiting oxygen concentrations resulted in an increase in respiratory activity, chemical energy content, and expression of the nitrogen-fixation gene nifHc. Expand
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