• Publications
  • Influence
Type VI collagen is a major component of the human cornea
Collagen type VI is shown to be present in the human cornea. This finding is based on comparative peptide mapping relative to type VI collagen isolated from placenta and on immunoblotting usingExpand
  • 113
  • 3
  • PDF
Type VI collagen is composed of a 200 kd subunit and two 140 kd subunits.
We have isolated type VI collagen, a transformation‐sensitive glycoprotein of the extracellular matrix, in an intact, disulfide‐bonded form. The protein contains a 200 kd subunit and two differentExpand
  • 76
  • 1
Basement membrane (type IV) collagen is a heteropolymer.
Type IV collagen was isolated in high yield from bovine kidney cortex. The protein revealed Mr = 380,000 and contained, in a 2:1 ratio, two different disulfide-linked polypeptide chains, C-1 and D-1Expand
  • 73
  • 1
Biosynthetic and structural properties of endothelial cell type VIII collagen.
A highly unusual endothelial cell collagen (Sage, H., Pritzl, P., and Bornstein, P., (1980) Biochemistry 19, 5747-5755) has been characterized in greater detail. Pulse-chase experiments with bovineExpand
  • 84
  • 1
Characterization of the precursor form of type VI collagen.
Well characterized monospecific antisera against pepsin-extracted bovine type VI collagen were used to identify and characterize the intact form of type VI collagen. In immunoblotting experiments theExpand
  • 48
Nonenzymatic glycosylation of proteins. A warning.
Radioactively labeled glucose from several manufacturers contains radioactive impurities of to date unknown structure. These contaminants bind covalently to proteins, e.g. collagen, fibronectin,Expand
  • 59
Nonenzymatic glycosylation of basement membrane collagen in diabetes mellitus.
For a better understanding of the processes leading to diabetic microangiopathy, type IV collagen from kidneys of patients with long-term diabetes was compared with the collagen from kidneys of sex-Expand
  • 41
Synthesis and quantitation of glucitollysine, a glycosylated amino acid elevated in proteins from diabetics.
Abstract A method for preparative synthesis of glucitollysine in high yield is described. It is shown that this compound is useful as a standard for the determination of nonenzymatic glycosylation ofExpand
  • 21
Monoclonal antibodies to human type IV collagen: useful reagents to demonstrate the heterotrimeric nature of the molecule.
Monoclonal antibodies (mAbs) have been prepared against type IV collagen isolated from human kidney. Two mAbs, designated CIV 22 and CIV 16, were extensively characterized. CIV 22 reacted only withExpand
  • 78
  • PDF
Type VI collagen represents a major fraction of connective tissue collagens.
A new method for the isolation of type VI collagen from peptic tissue digests is presented which gives tenfold higher yields than methods previously reported. From the amounts of purified proteinExpand
  • 73