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High-resolution in situ hybridization to whole-mount zebrafish embryos
TLDR
This protocol describes ISH of digoxigenin-labeled antisense RNA probes to whole-mount zebrafish embryos and uses conditions that favor specific hybridization to complementary mRNA sequences in the tissue(s) expressing the corresponding gene. Expand
A zebrafish nanos-related gene is essential for the development of primordial germ cells.
TLDR
It is found that several mechanisms act in concert to restrict the activity of Nanos1 to the germ cells including RNA localization and control over the stability and translatability of the RNA. Expand
dead end, a Novel Vertebrate Germ Plasm Component, Is Required for Zebrafish Primordial Germ Cell Migration and Survival
TLDR
It is shown that the RNA of dead end, encoding a novel putative RNA binding protein, is a component of the germ plasm in zebrafish and is specifically expressed in PGCs throughout embryogenesis; Dead End protein is localized to perinuclear germ granules within P GCs. Expand
Zebrafish early macrophages colonize cephalic mesenchyme and developing brain, retina, and epidermis through a M-CSF receptor-dependent invasive process.
TLDR
It is shown that a specific lineage of early macrophages that differentiate in the yolk sac before the onset of blood circulation spread in the whole cephalic mesenchyme, and from there invade epithelial tissues: epidermis, retina, and brain--especially the optic tectum. Expand
Structure of the zebrafish snail1 gene and its expression in wild-type, spadetail and no tail mutant embryos.
TLDR
The work presented here suggests that snail1 is involved in morphogenetic events during gastrulation, somitogenesis and development of the cephalic neural crest, and that no tail may act as a positive regulator of snail1. Expand
Ontogeny and behaviour of early macrophages in the zebrafish embryo.
TLDR
The results support the notion that in vertebrate embryos, macrophages endowed with proliferative capacity arise early from the hemopoietic lineage through a non-classical, rapid differentiation pathway, which bypasses the monocytic series that is well-documented in adult hemopOietic organs. Expand
Roles for Fgf signaling during zebrafish fin regeneration.
TLDR
It is found that fibroblast growth factor receptor 1 (fgfr1) is expressed in mesenchymal cells underlying the wound epidermis during blastema formation and in distal blastemal tissue during regenerative outgrowth and that a specific inhibitor of Fgfr1 applied immediately following fin amputation blocks Blastema formation, without obvious effects on wound healing. Expand
Antivin, a novel and divergent member of the TGFbeta superfamily, negatively regulates mesoderm induction.
TLDR
It is proposed that Antivin normally functions as a competitive inhibitor of Activin to limit mesoderm induction in the early embryo. Expand
Functions and regulations of fibroblast growth factor signaling during embryonic development.
Fibroblast growth factors (FGF) are secreted molecules which function through the activation of specific tyrosine kinases receptors, the FGF receptors that transduce the signal by activatingExpand
Sef is a feedback-induced antagonist of Ras/MAPK-mediated FGF signalling
TLDR
It is shown that in zebrafish, Sef functions as a feedback-induced antagonist of Ras/Raf/MEK/MAPK-mediated FGF signalling. Expand
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