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DNA Binding Specificity Studies of Four ETS Proteins Support an Indirect Read-out Mechanism of Protein-DNA Recognition* 210
TLDR
The results support a model in which cooperative effects among neighboring bases flanking the central G-G-A site contribute to the formation of stable ETS/DNA complexes, and are consistent with a mechanism for specific DNA binding that is partially governed by an indirect read-out of the DNA sequence.
Structure and function of the PWI motif: a novel nucleic acid-binding domain that facilitates pre-mRNA processing.
TLDR
It is shown here that the PWI motif is a new type of RNA/DNA-binding domain that has an equal preference for single- and double-stranded nucleic acids and likely has multiple important functions in pre-mRNA processing, including SRm160-dependent stimulation of 3'-end formation.
Pathway engineered enzymatic de novo purine nucleotide synthesis.
TLDR
A general method for isotopic labeling of the purine base moiety of nucleotides and RNA has been developed through biochemical pathway engineering in vitro, which permits complex synthetic cascades to be effected, expanding the applicability of enzymatic synthesis.
Enzymatic de novo pyrimidine nucleotide synthesis.
TLDR
De novo pyrimidine biosynthesis in vitro is recapitulate, using recombinantly expressed enzymes to perform efficient single-pot syntheses of UTP and CTP that bear a variety of stable isotope labeling patterns.
Enzymatic synthesis and structural characterization of 13C, 15N-poly(ADP-ribose).
TLDR
The labeled PAR synthesis reported here will provide an essential tool for the future study of PAR-protein complexes, and due to the extensive involvement of PAR in cell function and disease, further studies of PAR structure will be required.
Nitric-oxide Synthase Forms N-NO-pterin and S-NO-Cys
TLDR
Two specific non-heme iNOS nitrosation sites discovered by combining UV-visible spectroscopy, chemiluminescence, mass spectrometry, and x-ray crystallography are defined and suggest means for regulating iNos activity via N-NO-pterin and S- NO-Cys modifications.
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