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Relationship of sequence and structure to specificity in the α-amylase family of enzymes
The sequence- Specificity and structure-specificity relationships described may provide useful pointers for rational protein engineering. Expand
Protein engineering in the α-amylase family: catalytic mechanism, substrate specificity, and stability
  • B. Svensson
  • Biology, Medicine
  • Plant Molecular Biology
  • 1 May 1994
Prospects for rational engineering in this family include important members of plant origin, such as α-amylase, starch branching and debranching enzymes, and amylomaltase. Expand
Structure, specificity and function of cyclomaltodextrinase, a multispecific enzyme of the alpha-amylase family.
The present review discusses the multiple specificity in the light of the oligomerization and the molecular structures arriving at a clarified enzyme classification and a physiological role of the enzymes is proposed. Expand
Crystal and Molecular Structure of Barley α-Amylase
The three-dimensional structure of barley malt α-amylase (isoform AMY2-2) was determined by multiple isomorphous replacement using three heavy-atom derivatives and solvent flattening. The model wasExpand
Glucoamylase: structure/function relationships, and protein engineering.
Recent findings on the catalytic site, mechanism of action, substrate recognition, the linker region, the multidomain architecture, the engineering of specificity and stability, and roles of individual substrate binding subsites are focused on. Expand
α-Amylase: an enzyme specificity found in various families of glycoside hydrolases
α-Amylase is the main representative of family GH13, but it is probably also present in the families GH57 and GH119, and possibly even in GH126, and confirmation of the unambiguous presence of the α-amylase specificity may need more biochemical investigation. Expand
The carbohydrate‐binding module family 20 – diversity, structure, and function
The clear evolutionary relatedness of CBM20s to CBM21s, CBM48s and CBM53s suggests a common clan hosting most of the known starch‐binding domains, and some applications of SBDs as affinity or immobilization tags and, recently, in biofuel and in planta bioengineering are presented. Expand
The structure of barley alpha-amylase isozyme 1 reveals a novel role of domain C in substrate recognition and binding: a pair of sugar tongs.
A surface site recognizing maltooligosaccharides, not earlier reported for other alpha-amylases and probably associated with the different activity of AMY1 and AMY2 toward starch granules, has been identified and highlights a potential role of domain C. Expand