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Cis-active RNA elements (CREs) and picornavirus RNA replication.
The growing body of knowledge regarding picornavirus CREs is reviewed and how CRE RNAs work coordinately with viral replication proteins and other cis-active RNAs in the 5' and 3' NTRs during RNA replication is discussed. Expand
Poliovirus Polymerase Residue 5 Plays a Critical Role in Elongation Complex Stability
- Sarah E. Hobdey, B. J. Kempf, B. Steil, D. Barton, O. Peersen
- Biology, Medicine
- Journal of Virology
- 9 June 2010
Poliovirus polymerases with mutations of tryptophan 5 retain wild-type elongation rates, RNA binding affinities, and elongation complex formation rates but form unstable elongation complexes that reduce viral RNA production in a cell-free replication system. Expand
Replication of Poliovirus RNA with Complete Internal Ribosome Entry Site Deletions
Results suggest that RNA replication is not directly dependent on a template RNA first functioning as an mRNA, and further suggest that poliovirus RNA synthesis is not absolutely dependent on any protein-RNA interactions involving the IRES. Expand
Poliovirus cis-Acting Replication Element-Dependent VPg Uridylylation Lowers the Km of the Initiating Nucleoside Triphosphate for Viral RNA Replication
Low concentrations of UTP did not support negative-strand RNA synthesis when CRE-disrupting mutations prevented VPg uridylylation, whereas correspondingly low concentrations of CTP or GTP had no negative effects on the magnitude of CRE-independent negative-strate RNA synthesis. Expand
Conversion of VPg into VPgpUpUOH before and during Poliovirus Negative-Strand RNA Synthesis
The data implicate 2CATPase in the mechanisms whereby PV RNA functions as a template for reiterative CRE-dependent VPg uridylylation before and during negative-strand RNA synthesis. Expand
Antigen sparing with adjuvanted inactivated polio vaccine based on Sabin strains.
It is feasible to increase the potency of inactivated polio vaccines by using adjuvants, according to the results of a rat potency model. Expand
Poly(A) at the 3′ End of Positive-Strand RNA and VPg-Linked Poly(U) at the 5′ End of Negative-Strand RNA Are Reciprocal Templates during Replication of Poliovirus RNA
The data support a model of PV RNA replication wherein reiterative transcription of homopolymeric templates ensures the synthesis of long 3′ poly(A) tails on progeny RNA genomes. Expand
A mucosal adjuvant for the inactivated poliovirus vaccine.
- B. Steil, Patricia A Jorquera, J. Westdijk, W. Bakker, R. Johnston, M. Barro
- 23 January 2014
An IPV-GVI3000 vaccine would reduce the dose of IPV needed and provide significantly improved mucosal immunity and could be an effective tool to use in the poliovirus eradication campaign without risking the re-introduction of revertant poliov virus derived from OPV. Expand
Protein primers and a telomerase-like mechanism of poliovirus RNA replication maintain the 3' end of the RNA genome
- B. Steil