• Publications
  • Influence
Global mapping of translation initiation sites in mammalian cells at single-nucleotide resolution
Global translation initiation sequencing is presented, applying in parallel the ribosome E-site translation inhibitors lactimidomycin and cycloheximide to achieve simultaneous detection of both initiation and elongation events on a genome-wide scale. Expand
Decoding Human Cytomegalovirus
The results reveal an unanticipated complexity to the HCMV coding capacity and illustrate the role of regulated changes in transcript start sites in generating this complexity. Expand
Ribosomally synthesized and post-translationally modified peptide natural products: overview and recommendations for a universal nomenclature.
This review presents recommended nomenclature for the biosynthesis of ribosomally synthesized and post-translationally modified peptides (RiPPs), a rapidly growing class of natural products. TheExpand
Inhibition of Eukaryotic Translation Elongation by Cycloheximide and Lactimidomycin
A systematic comparative study of the effects of CHX and LTM on protein translation revealed both similarities and differences between the two inhibitors. Expand
Polyketide biosynthesis beyond the type I, II and III polyketide synthase paradigms.
  • B. Shen
  • Biology, Medicine
  • Current opinion in chemical biology
  • 1 April 2003
Examples of polyketide synthases with much greater diversity in both mechanism and structure than the current type I, II and III paradigms serve as an inspiration for searching novel polyketid synthases to give new insights intopolyketide biosynthesis and to provide new opportunities for combinatorial biosynthesis. Expand
Minimum Information about a Biosynthetic Gene cluster.
This work proposes the Minimum Information about a Biosynthetic Gene cluster (MIBiG) data standard, to facilitate consistent and systematic deposition and retrieval of data on biosynthetic gene clusters. Expand
Type I polyketide synthase requiring a discrete acyltransferase for polyketide biosynthesis
This work reports that the leinamycin biosynthetic gene cluster from Streptomyces atroolivaceus S-140 consists of two PKS genes, lnmI and lnmJ, that encode six PKS modules, none of which contain the cognate AT domain. Expand
Cloning and characterization of a phosphopantetheinyl transferase from Streptomyces verticillus ATCC15003, the producer of the hybrid peptide-polyketide antitumor drug bleomycin.
The development of a PCR method for cloning SFP-type PPTases from actinomycetes, the recognition of the Sfp-typePPTases to be associated with secondary metabolism with a relaxed carrier protein specificity, and the availability of Svp, in addition to Sfp, should facilitate future endeavors in engineered biosynthesis of peptide, polyketide, and, in particular, hybrid peptide-polyketide natural products. Expand
Hybrid peptide-polyketide natural products: biosynthesis and prospects toward engineering novel molecules.
It is shown that the same catalytic sites are conserved between the hybrid NRPS-PKS and normal NRPS or PKS systems, although the ketoacyl synthase domain in NRPS/PKS hybrids is unique, and that specific interpolypeptide linkers exist at both the C- and N-termini of the NRPS and PKS proteins. Expand
Tetracenomycin F1 monooxygenase: oxidation of a naphthacenone to a naphthacenequinone in the biosynthesis of tetracenomycin C in Streptomyces glaucescens.
The Tcm F1 monooxygenase apparently does not possess any of the prosthetic groups of known Monooxygenases, such as flavin or heme groups, nor does it utilize metal ions, suggesting that sulfhydryl groups and histidine residues are essential for the enzyme activity. Expand