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A sensitive genetic assay for the detection of cytosine deamination: determination of rate constants and the activation energy.
TLDR
The data suggest that agents which perturb DNA structure or facilitate direct protonation of cytosine may induce deamination at biologically significant rates, and the assay provides a means to directly test the hypothesis.
Conjugates of Antisense Oligonucleotides with the Tat and Antennapedia Cell-Penetrating Peptides: Effects on Cellular Uptake, Binding to Target Sequences, and Biologic Actions
TLDR
This assay clearly demonstrates the delivery of functional antisense molecules to the splicing machinery within the nucleus andjugation with cell-penetrating peptides enhances oligonucleotide delivery to the nucleus without interfering with the base-pairing function of antisense oligon nucleotides.
Cytosine deamination in mismatched base pairs.
TLDR
It is concluded that cytosine residues involved in a mispair in DNA are 1-2 orders of magnitude more prone to deaminate to uracil than are cytosines in double-stranded DNA.
Boron-containing aptamers to ATP.
TLDR
It is concluded that the bG and bU nucleotides are fully compatible with SELEX, and that these analogs could be used to make boronated aptamers as therapeutics for BNCT.
High potency silencing by single-stranded boranophosphate siRNA
TLDR
It is demonstrated that boranophosphate-modified ss-siRNA can silence gene expression as well as native ds-si RNA, suggesting thatboranoph phosphate- modified ss-SIRNAs should be investigated as a potential new class of therapeutic agents.
INCORPORATION OF RIBONUCLEOSIDE 5′-(α-P-BORANO)TRIPHOSPHATES INTO A 20-MER RNA BY T7 RNA POLYMERASE
  • Jing Wan, B. Shaw
  • Biology, Chemistry
    Nucleosides, nucleotides & nucleic acids
  • 1 April 2005
The enzymatic synthesis of short boranophosphate RNA was studied by comparing the yield and pattern of abortive products of in vitro transcription at steady-state conditions with that of normal RNA.
Analysis of subunit organization in chicken erythrocyte chromatin.
TLDR
Micrococcal nuclease digestion of intact chicken erythrocyte nuclei is shown to result in the formation of core nucleoprotein particles containing about 140 base pairs of DNA, indicating that the core particles the authors isolate are representative of nucleop protein structures existing within the nucleus.
Accelerated deamination of cytosine residues in UV-induced cyclobutane pyrimidine dimers leads to CC-->TT transitions.
TLDR
It is shown that the formation of pyrimidine dimers in DNA increases the rate of deamination by six orders of magnitude, leading to the accelerated formation of single C-->T and tandem double CC-->TT mutations.
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