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The virophage as a unique parasite of the giant mimivirus
TLDR
The Sputnik genome is an 18.343-kilobase circular double-stranded DNA and contains genes that are linked to viruses infecting each of the three domains of life Eukarya, Archaea and Bacteria and is classified as a virophage.
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A Giant Virus in Amoebae
During a study following a pneumonia outbreak in 1992, a microorganism growing in amoebae and resembling a small Gram-positive coccus ([Fig. 1][1]A) was isolated from the water of a cooling tower in…
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Culture of previously uncultured members of the human gut microbiota by culturomics
TLDR
It is shown that the use of culturomics allows the culture of organisms corresponding to sequences previously not assigned, which doubles the number of species isolated at least once from the human gut.
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“Megavirales”, a proposed new order for eukaryotic nucleocytoplasmic large DNA viruses
TLDR
These characteristics lead us to propose assigning an official taxonomic rank to the NCLDVs as the order “Megavirales”, in reference to the large size of the virions and genomes of these viruses.
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Modern clinical microbiology: new challenges and solutions
TLDR
New technologies, including mass spectrometry for colony identification, real-time genomics for isolate characterization, and versatile and permissive culture systems can improve the workflow and output of clinical microbiology laboratories and, by identifying and characterizing microbial pathogens, provide significant input to scientific discovery.
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Ultrastructural Characterization of the Giant Volcano-like Virus Factory of Acanthamoeba polyphaga Mimivirus
TLDR
The results showed that Mimivirus replication is an extremely efficient process that results from a rapid takeover of cellular machinery, and takes place in a unique and autonomous giant assembly centre, leading to the release of a large number of complex virions through amoebal lysis.
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MIMIVIRE is a defence system in mimivirus that confers resistance to virophage
TLDR
It is found that lineage A strains are resistant to Zamilon and contain the insertion of a repeated Zamilon sequence within an operon, here named the ‘mimivirus virophage resistance element’ (MIMIVIRE).
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Microbial diversity in the sputum of a cystic fibrosis patient studied with 16S rDNA pyrosequencing
TLDR
Compared to clonal Sanger sequencing and to cultivation results, pyrosequencing recovers greater species richness and gives a more reliable estimate of the relative abundance of bacterial species.
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Usefulness of rpoB Gene Sequencing for Identification of Afipia and Bosea Species, Including a Strategy for Choosing Discriminative Partial Sequences
TLDR
A simple computerized method that allows determination of nucleotide positions of high variability in the sequence that are bordered by conserved sequences and that could be useful for design of universal primers is proposed.
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Bartonella quintana in human erythrocytes
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