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Dynamic association of capping enzymes with transcribing RNA polymerase II.
The C-terminal heptad repeat domain (CTD) of RNA polymerase II (pol II) is proposed to target pre-mRNA processing enzymes to nascent pol II transcripts, but this idea has not been directly tested inExpand
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Prp43 Is an Essential RNA-dependent ATPase Required for Release of Lariat-Intron from the Spliceosome*
The essential Saccharomyces cerevisiae PRP43 gene encodes a 767-amino acid protein of the DEXH-box family. Prp43 has been implicated in spliceosome disassembly (Arenas, J. E., and Abelson, J. N.Expand
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Ntr1 activates the Prp43 helicase to trigger release of lariat-intron from the spliceosome.
DEAD/H-box NTPases remodel the spliceosome at multiple steps during the pre-mRNA splicing cycle. The RNA-dependent NTPase Prp43 catalyzes dissociation of excised lariat-intron from the spliceosome,Expand
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A conformational rearrangement in the spliceosome sets the stage for Prp22-dependent mRNA release.
  • B. Schwer
  • Biology, Medicine
  • Molecular cell
  • 20 June 2008
An essential step in pre-mRNA splicing is the release of the mRNA product from the spliceosome. The DEAH box RNA helicase Prp22 catalyzes mRNA release by remodeling contacts within the spliceosomeExpand
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Prp22, a DExH‐box RNA helicase, plays two distinct roles in yeast pre‐mRNA splicing
In order to assess the role of Prp22 in yeast pre‐mRNA splicing, we have purified the 130 kDa Prp22 protein and developed an in vitro depletion/reconstitution assay. We show that Prp22 is requiredExpand
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PRP16 is an RNA-dependent ATPase that interacts transiently with the spliceosome
The assembly of the spliceosome is an ATP-dependent process. The splicing factor PRP16 contains variations of several motifs that define the elF-4A-like ATP-dependent RNA helicase family. The proteinExpand
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How Slu7 and Prp18 cooperate in the second step of yeast pre-mRNA splicing.
Slu7 and Prp18 act in concert during the second step of yeast pre-mRNA splicing. Here we show that the 382-amino-acid Slu7 protein contains two functionally important domains: a zinc knuckleExpand
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RNA capping enzyme and DNA ligase: a superfamily of covalent nucleotidyl transferases
mRNA capping entails GMP transfer from GTP to a 5′ diphosphate RNA end to form the structure G(5′)ppp(5′)N. A similar reaction involving AMP transfer to the 5′ monophosphate end of DNA or RNA occursExpand
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TFIIH and P-TEFb coordinate transcription with capping enzyme recruitment at specific genes in fission yeast.
Cyclin-dependent kinases (CDKs) are subunits of transcription factor (TF) IIH and positive transcription elongation factor b (P-TEFb). To define their functions, we mutated the TFIIH-associatedExpand
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A conformational rearrangement in the spliceosome is dependent on PRP16 and ATP hydrolysis.
PRP16 is an RNA‐dependent ATPase that is required for the second catalytic step of pre‐mRNA splicing. We have previously shown that PRP16 protein binds stably to spliceosomes that have completed 5′Expand
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