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GREAT improves functional interpretation of cis-regulatory regions
We developed the Genomic Regions Enrichment of Annotations Tool (GREAT) to analyze the functional significance of cis-regulatory regions identified by localized measurements of DNA binding eventsExpand
Doublecortin Is a Developmentally Regulated, Microtubule-Associated Protein Expressed in Migrating and Differentiating Neurons
It is shown that Doublecortin is expressed in the brain throughout the period of corticogenesis in migrating and differentiating neurons, and Immunohistochemical studies show its localization in the soma and leading processes of tangentially migrating neuron, and a strong axonal labeling is observed in differentiating neuron. Expand
Characterization of the Kinetochore Binding Domain of CENP-E Reveals Interactions with the Kinetochore Proteins CENP-F and hBUBR1
As CENP-E and hBUBR1 can coimmunoprecipitate with each other from HeLa cells, they may function as a motor–kinase complex at kinetochores, however, the complex distribution pattern of hBubR1 suggests that it may regulate multiple functions that include the kinETochore and the spindle midzone. Expand
Cytoskeletal coordination during neuronal migration.
  • B. Schaar, S. Mcconnell
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences…
  • 20 September 2005
Time-lapse imaging and pharmacological perturbation suggest that nucleokinesis requires stepwise or hierarchical interactions between microtubules, myosin II, and cell adhesion, and it is hypothesized that these interactions couple leading process extension to nuclear translocation during neuronal migration. Expand
CENP-E is a putative kinetochore motor that accumulates just before mitosis
CENP-E is identified as a kinesin-like motor protein (Mr 312,000) that accumulates in the G2 phase of the cell cycle and is likely to be one of the motors responsible for mammalian chromosome movement and/or spindle elongation. Expand
CENP-E Function at Kinetochores Is Essential for Chromosome Alignment
The combined data show that CENP-E provides kinetochore functions that are essential for monopolar chromosomes to establish bipolar connections and for chromosomes with connections to both spindle poles to align at the spindle equator. Expand
Human-specific loss of regulatory DNA and the evolution of human-specific traits
510 complete deletion of sequences otherwise highly conserved between chimpanzees and other mammals are confirmed, which fall almost exclusively in non-coding regions and are enriched near genes involved in steroid hormone signalling and neural function. Expand
Doublecortin Microtubule Affinity Is Regulated by a Balance of Kinase and Phosphatase Activity at the Leading Edge of Migrating Neurons
Evidence that Dcx/microtubule interactions are negatively controlled by Protein Kinase A (PKA) and the MARK/PAR-1 family of protein kinases is presented and a serine within a novel sequence that is crucial for the PKA- and MARK-dependent regulation of DcX's microtubule binding activity in vitro is identified. Expand
Human neural stem cells enhance structural plasticity and axonal transport in the ischaemic brain
The results show the first evidence that human neural progenitor cell treatment can significantly increase dendritic plasticity in both the ipsi- and contralesional cortex and this coincides with stem cell-induced functional recovery, and postulate that human neurons aid recovery after stroke through secretion of factors that enhance brain repair and plasticity. Expand
Localization of CENP-E in the fibrous corona and outer plate of mammalian kinetochores from prometaphase through anaphase
The observations reported here are consistent with models in which CENP-E has a role in promoting the poleward migration of sister chromatids during anaphase A, and the normal disappearance of this Kinetochore substructure in conventional electron micrographs of mitotic chromosomes with attached kinetochores is not due to the corona becoming stretched along the spindle microtubules as has been suggested. Expand