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Structural Analysis of Histo-Blood Group Antigen Binding Specificity in a Norovirus GII.4 Epidemic Variant: Implications for Epochal Evolution
A plausible mechanism for how norovirus disassociates from salivary mucin-linked HBGA before reassociating with HBGAs linked to intestinal epithelial cells during its passage through the gastrointestinal tract is suggested. Expand
Structural basis of Fic mediated adenylylation
The structure of the second Fic domain of IbpA (IbpAFic2) in complex with its substrate, Cdc42 is presented, providing the first structural view for this post-translational modification. Expand
Structure and rearrangements in the carboxy-terminal region of SpIH channels.
A combination of X-ray crystallography and electrophysiology is used to study the allosteric mechanism for cAMP modulation of HCN channels and a mechanism for partial agonist action is elaborate that will likely extend to other cyclic nucleotide-regulated channels and enzymes. Expand
P-glycoprotein Is Stably Inhibited by Vanadate-induced Trapping of Nucleotide at a Single Catalytic Site (*)
The finding that vanadate trapping of nucleotide at just one site/Pgp is sufficient to give full inhibition of ATPase activity shows that the two predicted nucleotide sites can not function independently as catalytic sites. Expand
The Influenza A Virus Protein NS1 Displays Structural Polymorphism
ABSTRACT NS1 of influenza A virus is a potent antagonist of host antiviral interferon responses. This multifunctional protein with two distinctive domains, an RNA-binding domain (RBD) and an effectorExpand
Structure and function of the Zika virus full-length NS5 protein
The crystal structures of full-length NS5 and its polymerase domain at 3.0 Å resolution are reported, which will contribute to future studies on ZikV infection and the development of inhibitors of ZIKV replication. Expand
Both P-glycoprotein Nucleotide-binding Sites Are Catalytically Active (*)
The inhibited P-glycoprotein·Mg-nucleotide·vanadate complex is probably an analog of the catalytic transition state, implying that when one nucleotide site assumes the catalytical transition state conformation the other site cannot do so and suggesting that the two sites may alternate in catalysis. Expand
Cysteine dioxygenase structures from pH4 to 9: consistent cys-persulfenate formation at intermediate pH and a Cys-bound enzyme at higher pH.
High-resolution structures of CDO soaked with Cys at pH values from 4 to 9 imply that an iron-bound persulfenate (or persulfenic acid) is energetically accessible in the CDO active site, and thatCDO active-site chemistry in the crystals is influenced by protonation/deprotonation events with effective pKa values near ~5.5 and ~7.5 that influence Cys binding and oxygen binding/reactivity, respectively. Expand
Mechanism of TRIM25 Catalytic Activation in the Antiviral RIG-I Pathway
It is shown that the RING domain is a separate self-association motif that engages ubiquitin-conjugated E2 enzymes as a dimer that helps to fine-tune the efficiency of the RIG-I-mediated antiviral response. Expand
Crystal structure of cGMP-dependent protein kinase reveals novel site of interchain communication.
The 2.5 Å crystal structure of a regulatory domain construct containing both cGMP binding sites of PKG Iα and a previously uncharacterized helical domain (switch helix) is reported, offering new structural insight into the mechanism of allosteric PKG activation. Expand