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Ligand recognition and gating mechanism through three ligand-binding sites of human TRPM2 channel
TRPM2 is critically involved in diverse physiological processes including core temperature sensing, apoptosis, and immune response. TRPM2’s activation by Ca2+ and ADP ribose (ADPR), anExpand
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Monoclonal antibodies to the main immunogenic region of the nicotinic acetylcholine receptor bind to residues 61-76 of the alpha subunit.
Monoclonal antibodies (mAbs) to the main immunogenic region (MIR) bind to fusion proteins containing region 37-200 of the alpha chain of Torpedo, mouse, and chicken nicotinic acetylcholine receptor.Expand
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Mapping the main immunogenic region and toxin-binding site of the nicotinic acetylcholine receptor.
The alpha-chain of the nicotinic acetylcholine receptor carries the binding sites both for cholinergic ligands and for most experimentally induced or naturally occurring antibodies to the nativeExpand
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A modified nicotinic acetylcholine receptor lacking the ‘ion channel amphipathic helices’
Antibodies to a synthetic peptide from the ‘amphipathic helix’ of the α‐chain of the nicotinic acetylcholine receptor (nAChR) bound both to detergent‐solubilised and membrane‐bound nAChR, indicatingExpand
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Localization of the Main Immunogenic Region and Toxin Binding Site of the Nicotinic Acetylcholine Receptor a
We have used two approaches in order to map the main immunogenic region (MIR) and the a-bungarotoxin (aBTX) binding site of the nicotinic acetylcholine receptor (nAChR). Digestion of Torpedo nAChR byExpand
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Expression Cloning and Fusion Proteins as Tools to Study Receptor Structure
The nicotinic acetylcholine receptor (nAChR) is a complex molecule consisting of four different subunits α, β, γ and δ which form a pentameric structure containing two copies of the α-chain and oneExpand
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Ammonium sulphate precipitation overestimates titres of anti-peptide antisera.
Precipitation by 50% final saturation of ammonium sulphate, a method commonly used to estimate titres and affinity constants of antibodies, was found consistently to overestimate the titre ofExpand
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