Unresponsiveness to cannabinoids and reduced addictive effects of opiates in CB1 receptor knockout mice.
Observations suggest that the CB1 receptor is involved in the motivational properties of opiates and in the development of physical dependence and extend the concept of an interconnected role of CB1 and opiate receptors in the brain areas mediating addictive behavior.
Loss of morphine-induced analgesia, reward effect and withdrawal symptoms in mice lacking the µ-opioid-receptor gene
Investigation of the behavioural effects of morphine reveals that a lack of μ receptors abolishes the analgesic effect of morphine, as well as place-preference activity and physical dependence, and concludes that the µ-opioid-receptor gene product is a mandatory component of the opioid system for morphine action.
The HIV-1 Viral Protein R Induces Apoptosis via a Direct Effect on the Mitochondrial Permeability Transition Pore
Coincubation of purified organelles revealed that nuclear apoptosis is only induced by Vpr when mitochondria are present yet can be abolished by PTPC inhibitors, suggesting that Vpr induces apoptosis via a direct effect on the mitochondrial PTPC.
Neutral endopeptidase 24.11: structure, inhibition, and experimental and clinical pharmacology.
International Union of Pharmacology. XXI. Structure, distribution, and functions of cholecystokinin receptors.
The peptide cholecystokinin has been shown to mediate pancreatic secretion and contraction of gallbladder and was described in the mammalian central nervous system (CNS) as a gastrin-like peptide.
Control of Mitochondrial Membrane Permeabilization by Adenine Nucleotide Translocator Interacting with HIV-1 Viral Protein R and Bcl-2
It is demonstrated that a synthetic Vpr-derived peptide specifically binds to the intermembrane face of the ANT with an affinity in the nanomolar range, which indicates that both Vpr and Bcl-2 modulate MMP through a direct interaction with ANT.
Structure of the winged-helix protein hRFX1 reveals a new mode of DNA binding
A 1.5 Å-resolution structure of two copies of the DBD of human RFX1 (hRFX1) binding cooperatively to a symmetrical X-box is presented and a new model for interactions between linker histones and DNA is proposed.
NMR structure of the HIV-1 regulatory protein VPR.
First glimpses at structure-function relationships of the nucleocapsid protein of retroviruses.
- J. Darlix, M. Lapadat-Tapolsky, H. de Rocquigny, B. Roques
- BiologyJournal of Molecular Biology
- 8 December 1995
The 3-D structures of HIV-1 and MoMuLV NC proteins, deduced from NMR studies, are characterized by a central globular domain with one (MoMuLV) or two (HIV-1) zinc fingers, which should facilitate a rational approach of new anti-HIV therapies based on inhibition of NC protein functions.
Viral RNA annealing activities of human immunodeficiency virus type 1 nucleocapsid protein require only peptide domains outside the zinc fingers.
- H. de Rocquigny, C. Gabus, A. Vincent, M. Fournié-Zaluski, B. Roques, J. Darlix
- Biology, ChemistryProceedings of the National Academy of Sciences…
- 15 July 1992
It is shown here that synthetic NCp7 with or without the two zinc fingers has the RNA annealing activities of NCp15, and could be used as a model for the design of antiviral agents.