B. R. Leeflang

Publications50
h-index23
Citations1,571
Highly Influential Citations33
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Structural characterisation of the exopolysaccharide produced by Lactobacillus delbrückii subspecies bulgaricus rr grown in skimmed milk.
The exopolysaccharide of Lactobacillus delbrückii subsp. bulgaricus rr, isolated from skimmed milk, is a heteropolymer of D-galactopyranosyl, D-glucopyranosyl, and L-rhamnopyranosyl residues in the… Expand
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Development of a 1H NMR structural-reporter-group concept for the primary structural characterisation of alpha-D-glucans.
An NMR study of proton chemical shift patterns of known linear alpha-D-glucopyranose di- and trisaccharide structures was carried out. Chemical shift patterns for (alpha1-->2)-, (alpha1-->3)-,… Expand
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The Giardia intestinalis filamentous cyst wall contains a novel beta(1-3)-N-acetyl-D-galactosamine polymer: a structural and conformational study.
Assembly of a protective cyst wall by Giardia is essential for the survival of the parasite outside the host intestine and for transmission among susceptible hosts. The structure of the G.… Expand
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Characterisation by 1H-n.m.r. spectroscopy of oligosaccharides, derived from arabinoxylans of white endosperm of wheat, that contain the elements →4)[α-l-Araf-(1-ar3)]-β-d-Xylp-(1→ or…
Abstract The structure of penta- to hepta-saccharides, generated by digestion of purified wheat-endosperm arabinoxylan with endo-(1→4)-β- d -xylanase and isolated by gel-permeation chromatography on… Expand
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Structure of the exopolysaccharide produced by Lactococcus lactis subspecies cremoris H414 grown in a defined medium or skimmed milk.
The structure of the exopolysaccharide of Lactococcus lactis subsp. cremoris H414, isolated from a defined medium or skimmed milk, was established by linkage analysis on the native polysaccharide,… Expand
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Cyclogossine A: a novel cyclic heptapeptide isolated from the latex of Jatropha gossypifolia.
From the latex of Jatropha gossypifolia L. (Euphorbiaceae) a novel cyclic heptapeptide was isolated, which we named cyclogossine A. A combination of amino acid analysis, FAB mass spectrometry, and… Expand
  • 45
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High-level expression of biologically active glycoprotein hormones in Pichia pastoris strains—selection of strain GS115, and not X-33, for the production of biologically active N-glycosylated…
The methylotrophic yeast Pichia pastoris is widely used for the production of recombinant glycoproteins. With the aim to generate biologically active 15N-labeled glycohormones for conformational… Expand
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NMR spectroscopy of nystose.
Sucrose (11, 1-kestose (21, and nystose (3) are the smallest inulin-related oligosaccharides1T2, which consist of (2 -+ l)-linked /3-D-fructofuranosyl residues, terminated by an a-D-glucopyranosyl… Expand
  • 21
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CROSREL: Full relaxation matrix analysis for NOESY and ROESY NMR spectroscopy
SummaryA method is proposed for quantitative analysis of ROESY peak intensities, to which corrections are applied for their offset dependence and for direct HOHAHA effects. Additionally the effects… Expand
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Characterization of the N-linked oligosaccharides from human chorionic gonadotropin expressed in the methylotrophic yeast Pichia pastoris
Human chorionic gonadotropin (hCG) is a heterodimeric, placental glycoprotein hormone involved in the maintenance of the corpus luteum during the first trimester of pregnancy. Biologically active hCG… Expand
  • 32
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