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ADP Is the Cognate Ligand for the Orphan G Protein-coupled Receptor SP1999*
  • Fang L. Zhang, Lin Luo, +10 authors F. Monsma
  • Biology, Medicine
    The Journal of Biological Chemistry
  • 16 March 2001
It is demonstrated that SP1999 is a Gαi-coupled receptor that is potently activated by ADP, and is a novel, G αi-linked receptor for ADP.
Mass spectrometric identification of a naturally processed melanoma peptide recognized by CD8+ cytotoxic T lymphocytes
P939 represents a good candidate for the development of peptide-based immunotherapies for the treatment of patients with melanoma and is coordinately recognized by a CTL clone obtained by limiting dilution from the peripheral blood of an HLA- A2+ melanoma patient.
Matrix Dependence of Metastable Fragmentation of Glycoproteins in MALDI TOF Mass Spectrometry
Matrix-assisted laser desorption ionization (MALDI) mass spectrometry relies on the formation of intact molecular ions in order to achieve a high-accuracy molecular weight determination. Problems
Ras oncoprotein inhibitors: the discovery of potent, ras nucleotide exchange inhibitors and the structural determination of a drug-protein complex.
The development and use of mass spectrometry, NMR spectroscopy and molecular modeling techniques are described to elucidate the structure of a drug-protein complex, and aid in designing new ras inhibitor targets.
Application of LC/MS to proteomics studies: current status and future prospects.
Current LC/MS approaches in protein characterization, including a bottom-up method for protein identification and quantitative proteomics, are described, including the investigation of protein post-translational modifications such as glycosylation (glycoproteomics) and phosphorylation (phosphoproteomics).
Applications of LC/MS in structure identifications of small molecules and proteins in drug discovery.
The role of LC/MS analysis in drug discovery process will be illustrated with examples on high-throughput analysis, structural analysis of trace level impurities in drug substances, identification of metabolites, and characterization of therapeutic protein products for process improvement.
Arachidonic acid epoxygenase: detection of epoxyeicosatrienoic acids in human urine.
Epoxyeicosatrienoic acids, metabolites of the cytochrome P-450-mediated epoxygenase reaction, were detected in human urine by gas chromatographic-mass spectroscopic techniques after conversion to
Electrospray ionization mass spectrometry for the study of non-covalent complexes: an emerging technology.
The usefulness of ESI-MS for addressing biological problems leading to the discovery of new therapeutics is illustrated; the approach involves the rapid screening of potential drug candidates, such as weakly bound inhibitors.
Applied electrospray mass spectrometry
Historical review/principles/instrumentation nano-spray ESI development - LC/MS, CE/MS application antibiotics/pharmaceuticals pharmacokinetics/drug metabolism proteins/peptides glyco-proteins
Saponins from Collinsonia canadensis
The structure of collinsonin has been established as 3-O-α-L-arabinopyranosylcollin sogenin, and two new saponins named collinsogenin and collinsonidin were isolated from the roots of Collinsonia canadensis.