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Over-production of proteins in Escherichia coli: mutant hosts that allow synthesis of some membrane proteins and globular proteins at high levels.
Investigation of the over-production of seven membrane proteins in an Escherichia coli-bacteriophage T7 RNA polymerase expression system found that when expression of the target membrane protein was induced, most of the BL21(DE3) host cells died.
Disruption of the uncoupling protein-2 gene in mice reveals a role in immunity and reactive oxygen species production
It is demonstrated here that mice lacking Ucp2 following targeted gene disruption are not obese and have a normal response to cold exposure or high-fat diet and a role for UCP2 in the limitation of ROS and macrophage-mediated immunity is indicated.
Uncoupling Protein 2, in Vivo Distribution, Induction upon Oxidative Stress, and Evidence for Translational Regulation*
It is shown that an upstream open reading frame located in exon two of theucp2 gene strongly inhibits the expression of the protein, which provides a mechanism by which expression can be strongly and rapidly induced under stress conditions.
The biology of mitochondrial uncoupling proteins.
In comparison to the established uncoupling and thermogenic activities of UCP1, UCP2 and UCP3 appear to be involved in the limitation of free radical levels in cells rather than in physiological uncouplings and thermogenesis.
BMCP1, a Novel Mitochondrial Carrier with High Expression in the Central Nervous System of Humans and Rodents, and Respiration Uncoupling Activity in Recombinant Yeast*
Cl cloning and functional analysis of a novel homologue of the mitochondrial carriers predominantly expressed in the central nervous system and referred to as BMCP1 (brain mitochondrialcarrier protein-1) showed that mRNA of this novel gene is mainly expressed in brain, although it is 10–30-fold less expressed in other tissues.
Uncoupling protein‐2 controls proliferation by promoting fatty acid oxidation and limiting glycolysis‐derived pyruvate utilization
- C. Pecqueur, Thi V Bui, C. Thompson
- BiologyFASEB journal : official publication of the…
- 1 January 2008
It is shown that loss of function of UCP2 does not result in a significant increase in ROS production or an increased propensity for cells to undergo senescence in culture, and instead, Ucp2—/— cells display enhanced proliferation associated with a metabolic switch from fatty acid oxidation to glucose metabolism.
Characterisation of new intracellular membranes in Escherichia coli accompanying large scale over‐production of the b subunit of F1Fo ATP synthase
ATP synthase from bovine heart mitochondria. In vitro assembly of a stalk complex in the presence of F1-ATPase and in its absence.
- I. Collinson, M. V. van Raaij, J. Walker
- Biology, ChemistryJournal of molecular biology
- 29 September 1994
It was demonstrated that the binding of the various subunits to F1-ATPase increases the ATP hydrolase activity and diminishes its inactivation by exposure to cold, and these assembly experiments help to define some of the inter-subunit interactions in the stalk region of the F1F0- ATPase complex.
Dangerous liaisons between detergents and membrane proteins. The case of mitochondrial uncoupling protein 2.
How dodecylphosphocholine severely alters the structure as well as the function of UCPs is demonstrated, opening new vistas for probing the physiological relevance of three-dimensional structures of membrane proteins obtained in non-native environments.
Bone Marrow Transplantation Reveals the in Vivo Expression of the Mitochondrial Uncoupling Protein 2 in Immune and Nonimmune Cells during Inflammation*
- M. Alves-Guerra, S. Rousset, B. Miroux
- Biology, MedicineJournal of Biological Chemistry
- 24 October 2003
It is shown that there is down-regulation of UCP2 in immune cells during their activation in the early stages of the LPS response followed by an up-regulation in U CP2 during the later stages to protect all cells against oxidative stress.