B. Middleton

Publications108
h-index23
Citations1,677
Highly Influential Citations36
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The oxoacyl-coenzyme A thiolases of animal tissues.
  • B. Middleton
  • Biology, Medicine
  • The Biochemical journal
  • 1 April 1973
1. The activities and relative 3-oxoacyl-CoA substrate specificities of oxoacyl-CoA thiolase were determined in a large number of animal tissues. The relative activities with different 3-oxoacyl-CoA… Expand
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Modulating the potency of an activator in a yeast in vitro transcription system.
The intrinsic stimulatory potential or potency of a eukaryotic gene activator is controlled by the interaction between the activation domain and the transcriptional machinery. To further understand… Expand
  • 47
  • 6
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Human liver long-chain 3-hydroxyacyl-coenzyme A dehydrogenase is a multifunctional membrane-bound beta-oxidation enzyme of mitochondria.
We have purified to homogeneity the long-chain specific 3-hydroxyacyl-CoA dehydrogenase from mitochondrial membranes of human infant liver. The enzyme is composed of non-identical subunits of 71 kDa… Expand
  • 132
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The synthesis and characterisation of 2-methylacetoacetyl coenzyme A and its use in the identification of the site of the defect in 2-methylacetoacetic and 2-methyl-3-hydroxybutyric aciduria.
(1) 2-Methylacetoacetyl coenzyme A was prepared, purified by ion exchange chromatography as judged by TLC and HPLC and a number of its properties characterised. (2) An assay of 3-oxothiolase in… Expand
  • 77
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3-Ketoacyl-CoA thiolases of mammalian tissues.
Publisher Summary This chapter discusses the determination of 3-ketoacyl-CoA thiolases of mammalian tissues. 3-Ketoacyl-CoA thiolase activity is determined by following the stimulation of… Expand
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Mitochondrial Fatty Acid β-Oxidation in the Human Eye and Brain: Implications for the Retinopathy of Long-Chain 3-Hydroxyacyl-CoA Dehydrogenase Deficiency
The retinal pigment epithelium (RPE) and the choriocapillaris are affected early in the retinopathy associated with long-chain 3-hydroxyacyl-CoA dehydrogenase (LCHAD) deficiency. RPE in culture… Expand
  • 57
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Urinary excretion of 2-methylacetoacetate, 2-methyl-3-hydroxybutyrate and tiglylglycine after isoleucine loading in the diagnosis of 2-methylacetoacetyl-CoA thiolase deficiency
SummaryThe concentrations of 2-methylacetoacetate, 2-methyl-3-hydroxybutyrate and tiglylglycine were determined by gas chromatography-mass spectrometry in urine collected before and for 8 h after… Expand
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Effects of hypothyroidism and high-fat feeding on mRNA concentrations for the low-density-lipoprotein receptor and on acyl-CoA:cholesterol acyltransferase activities in rat liver.
1. Induction of hypothyroidism in rats by feeding propylthiouracil (PTU) significantly increased serum cholesterol concentrations, and the effect was more pronounced for cholesterol in low-density… Expand
  • 57
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The kinetic mechanism and properties of the cytoplasmic acetoacetyl-coenzyme A thiolase from rat liver.
  • B. Middleton
  • Chemistry, Medicine
  • The Biochemical journal
  • 1 April 1974
1. Cytoplasmic acetoacetyl-CoA thiolase was highly purified in good yield from rat liver extracts. 2. Mg(2+) inhibits the rate of acetoacetyl-CoA thiolysis but not the rate of synthesis of… Expand
  • 59
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The kinetic mechanism of 3-hydroxy-3-methylglutaryl-coenzyme A synthase from baker's yeast.
1. The effect of independent variation of both acetyl-CoA and acetoacetyl-CoA on the initial velocity at pH8.0 and pH8.9 gives results compatible with a sequential mechanism involving a modified… Expand
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