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Superoxide generation by endothelial nitric oxide synthase: the influence of cofactors.
Results demonstrate that superoxide is generated from the oxygenase domain by dissociation of the ferrous-dioxygen complex and that occupation of the L-arginine binding site does not inhibit this process, and indicate that modulation of BH4 concentration may regulate the ratio of superoxide to nitric oxide generated by eNOS. Expand
Three-dimensional structure of NADPH-cytochrome P450 reductase: prototype for FMN- and FAD-containing enzymes.
The structure of rat liver CPR, expressed in Escherichia coli and solubilized by limited trypsinolysis, has been determined by x-ray crystallography at 2.6 A resolution. Expand
Dissecting the Interaction between Nitric Oxide Synthase (NOS) and Caveolin
The data demonstrate a novel functional role for Caveolin-1 in mammalian cells as a potential molecular chaperone that directly inactivates NOS and the inactivation of eNOS and nNOS by the scaffolding domain of caveolin-3 suggests that eN OS in cardiac myocytes and n NOS in skeletal muscle are likely subject to negative regulation by this muscle-specific caveolin isoform. Expand
Crystal Structure of Constitutive Endothelial Nitric Oxide Synthase A Paradigm for Pterin Function Involving a Novel Metal Center
The unexpected recognition of the substrate, L-arginine, at the H4B site indicates that this site is poised to stabilize a positively charged pterin ring and suggests a model involving a cationic pterIn radical in the catalytic cycle. Expand
Some properties of a detergent-solubilized NADPH-cytochrome c(cytochrome P-450) reductase purified by biospecific affinity chromatography.
Titration of these purified preparations aerobically with both NADPH and potassium ferricyanide demonstrated unequivocally that the air-stable, reduced form of NADPH-cytochrome c (P-450) reductase contains 2 electron equivalents, confirming recent results obtained. Expand
Effects of 17-octadecynoic acid, a suicide-substrate inhibitor of cytochrome P450 fatty acid omega-hydroxylase, on renal function in rats.
The results suggest that endogenous cytochrome P450 metabolites of arachidonic acid influence renal medullary hemodynamics and the excretion of water and electrolytes. Expand
Endothelial nitric oxide synthase-dependent superoxide generation from adriamycin.
It is demonstrated here that the endothelial isoform of nitric oxide synthase (eNOS) reduces adriamycin to the semiquinone radical, which enhances superoxide formation and Nitric oxide production and leads eNOS to generate peroxynitrite and hydrogen peroxide, potent oxidants implicated in several vascular pathologies. Expand
Structural basis for human NADPH-cytochrome P450 oxidoreductase deficiency
CYPOR dysfunction in patients harboring these particular mutations may possibly be prevented by riboflavin therapy in utero, if predicted prenatally, or rescued postnatally in less severe cases. Expand
20-Hydroxyeicosatetraenoic acid is an endogenous vasoconstrictor of canine renal arcuate arteries.
The results indicate that 20-HETE is an endogenous constrictor of canine renal arcuate arteries and suggests a potential role for this substance in the regulation of renal vascular tone. Expand
Intrinsic and extrinsic modulation of nitric oxide synthase activity.