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Multicenter Validation of the Analytical Accuracy of Salmonella PCR: towards an International Standard
ABSTRACT As part of a major international project for the validation and standardization of PCR for detection of five major food-borne pathogens, four primer sets specific for Salmonella species wereExpand
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Practical Considerations in Design of Internal Amplification Controls for Diagnostic PCR Assays
The explosive increase since the beginning of 1990s in the number of publications reporting PCR-based methods for detection or molecular typing of food-borne pathogens has attracted the attention ofExpand
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Phenotypic and genotypic characterization of antimicrobial resistance in German Escherichia coli isolates from cattle, swine and poultry.
OBJECTIVE Phenotypic and genotypic characterization of the antimicrobial resistance of German Escherichia coli strains isolated during 1999-2001 from cattle, swine and poultry. MATERIALS ANDExpand
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Standardization of diagnostic PCR for the detection of foodborne pathogens.
In vitro amplification of nucleic acids using the polymerase chain reaction (PCR) has become, since its discovery in the 1980s, a powerful diagnostic tool for the analysis of microbial infections asExpand
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Diagnostic Real-Time PCR for Detection of Salmonella in Food
ABSTRACT A robust 5′ nuclease (TaqMan) real-time PCR was developed and validated in-house for the specific detection of Salmonella in food. The assay used specifically designed primers and a probeExpand
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Pork Contaminated with Salmonellaenterica Serovar 4,[5],12:i:−, an Emerging Health Risk for Humans
ABSTRACT Salmonella enterica subsp. enterica serovar 4,[5],12:i:− is a monophasic variant of S. enterica serovar Typhimurium (antigenic formula 4,[5],12:i:1,2). Worldwide, especially in severalExpand
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Enumeration of Salmonella Bacteria in Food and Feed Samples by Real-Time PCR for Quantitative Microbial Risk Assessment
Quantitative microbial risk assessment (QMRA) is an important approach for food safety in which risk and factors that influence food safety are identified. The goal is to provide an estimate of theExpand
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Rapid Classification and Identification of Salmonellae at the Species and Subspecies Levels by Whole-Cell Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry
ABSTRACT Variations in the mass spectral profiles of multiple housekeeping proteins of 126 strains representing Salmonella enterica subsp. enterica (subspecies I), S. enterica subsp. salamaeExpand
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A real-time PCR for the detection of Salmonella Enteritidis in poultry meat and consumption eggs.
A robust duplex 5' nuclease (TaqMan) real-time PCR was developed and in-house validated for the specific detection of Salmonella enterica subspecies enterica serovar Enteritidis in whole chickenExpand
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Interlaboratory diagnostic accuracy of a Salmonella specific PCR-based method.
A collaborative study involving four European laboratories was conducted to investigate the diagnostic accuracy of a Salmonella specific PCR-based method, which was evaluated within the EuropeanExpand
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