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Structure of baby hamster kidney carbohydrate-binding protein CBP30, an S-type animal lectin.
Physical measurements using CD and tryptophan fluorescence spectroscopy indicate that the two domains of CBP30 are structurally, as well as functionally, distinct and independent and cross-linking studies indicate thatThe amino-terminal lectin fragment can efficiently self-assemble into oligomeric species, and less efficient but significant aggregation of the intact lectin is also shown. Expand
Plasma membrane targetting, vesicular budding and release of galectin 3 from the cytoplasm of mammalian cells during secretion.
It is shown here that hamster galectin 3 overexpressed in transfected cos-7 cells is secreted at a very low rate indicating that movement of cytoplasmic galactoside-binding protein to plasma membrane domains is a rate limiting step in lectin secretion. Expand
Identification and characterization of a novel retroviral-like aspartic protease specifically expressed in human epidermis.
Indinavir, a potent HIV protease inhibitor used in AIDS therapy, had a significant inhibitory effect on rSASPase auto-activation, which could explain its side effects on skin. Expand
Retargeting of cytosolic proteins to the plasma membrane by the Lck protein tyrosine kinase dual acylation motif.
It is shown that chimaeras of the Lck dual acylation motif with two normally cytosolic proteins (chloramphenicol acetyl transferase and galectin-3) are resistant to extraction with cold non-ionic detergent, indicating targeting to GEM subdomains in the plasma membrane. Expand
Transcriptional Analysis of Vitiligo Skin Reveals the Alteration of WNT Pathway: A Promising Target for Repigmenting Vitiligo Patients.
The results shed light on the previously unrecognized role of decreased WNT activation in the prevention of melanocyte differentiation in depigmented vitiligo skin and support further clinical exploration of WNT agonists to repigment Vitiligo lesions. Expand
Identification and Cloning of a New Calmodulin-like Protein from Human Epidermis*
After separating by two-dimensional gel electrophoresis an extract of total proteins from human stratum corneum, two spots were extracted and analyzed for their peptide sequence. The resultingExpand
Nuclear localisation of wild type and mutant galectin‐3 in transfected cells
It is described that Cos‐7 cells or rabbit smooth muscle Rb‐1 cells transfected with cDNA encoding hamster galectin‐3 sequester the protein in nuclei whereas untransfected BHK cells expressing the endogenous hamster lectin or transfects over‐expressing the protein, do not. Expand
Analysis of proteins with caseinolytic activity in a human stratum corneum extract revealed a yet unidentified cysteine protease and identified the so-called "stratum corneum thiol protease" as
The results indicate that the stratum corneum thiol protease is probably expressed as a pro-enzyme in the lower layers of the epidermis and in part activated by a yet unidentified mechanism in the upper layers during keratinocyte differentiation, in contrast to earlier reports. Expand
IL-17/Th17 Pathway Is Activated in Acne Lesions
The presence of IL-17A positive T cells and the activation of Th17-related cytokines in acne lesions are demonstrated, indicating that the Th17 pathway is activated and may play a pivotal role in the disease process, possibly offering new targets of therapy. Expand
Calmodulin-like skin protein: a new marker of keratinocyte differentiation.
Calmodulin-like skin protein is a new marker of late keratinocyte differentiation with a role distinct from calmodulin, a recently discovered new skin-specific calcium binding protein that is expressed throughout the living layers of the epidermis. Expand