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High Resolution Reaction Intermediates of Rabbit Muscle Fructose-1,6-bisphosphate Aldolase
TLDR
The multifunctional role of Glu-187 epitomizes a canonical mechanistic feature conserved in Schiff base-forming aldolases catalyzing carbohydrate metabolism, and shows stereospecific discrimination and reduced ketohexose binding specificity.
Structure of a Class I Tagatose-1,6-bisphosphate Aldolase
TLDR
Structural analysis indicates extensive active site conservation with respect to class I FBP aldolases, including conserved conformational responses to DHAP binding and conserved stereospecific proton transfer at the DHAP C3 carbon mediated by a proximal water molecule.
Purification, crystallization and preliminary X-ray analysis of native and selenomethionine class I tagatose-1,6-bisphosphate aldolase from Streptococcus pyogenes.
  • B. Liotard, J. Sygusch
  • Biology, Medicine
    Acta crystallographica. Section D, Biological…
  • 1 March 2004
TLDR
The recombinant protein from Streptococcus pyogenes was overexpressed in Escherichia coli in its native and selenomethionine-derivative forms and purified using ion-exchange and hydrophobic interaction chromatography, obtaining orthorhombic crystals suitable for structural analysis.
1 STRUCTURE OF A CLASS I TAGATOSE-1 , 6-BIPHOSPHATE ALDOLASE : STUDY INTO AN APPARENT LOSS OF STEREOSPECIFICITY
Tagatose-1,6-biphosphate (TBP) aldolase from Streptococcus pyogenes is a class I aldolase that exhibits a remarkable lack of chiral discrimination with respect to the configuration of hydroxyl groups