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Complete Replication of Hepatitis C Virus in Cell Culture
A full-length HCV genome that replicates and produces virus particles that are infectious in cell culture (HCVcc) is described, suggesting that this in vitro system will aid in the search for improved antiviral compounds.
Identification of microRNAs of the herpesvirus family
To identify other miRNA genes in pathogenic viruses, a new miRNA gene prediction method with small-RNA cloning from several virus-infected cell types was combined and predicted miRNAs in several large DNA viruses.
Unravelling hepatitis C virus replication from genome to function
Since the discovery of the hepatitis C virus over 15 years ago, scientists have raced to develop diagnostics, study the virus and find new therapies. Yet virtually every attempt to dissect this
Cellular cofactors affecting hepatitis C virus infection and replication
The data define 26 host genes that modulate HCV infection and indicate that the requirement for functional RNAi for HCV replication is dominant over any antiviral activity this pathway may exert against HCV.
Genetic Interaction of Flavivirus Nonstructural Proteins NS1 and NS4A as a Determinant of Replicase Function
It is reported that the NS1 gene from a related virus, dengue virus (DEN), is unable to function in the process of YF RNA replication, suggesting a potential mechanism for integrating NS1 into the cytoplasmic process of RNA replication.
Structural Insights into RNA Recognition by RIG-I
The crystal structure of RIG-I in complex with double-stranded RNA reveals complex interplay between motor domains, accessory mechanical domains, and RNA that has implications for understanding the nanomechanical function of this protein family and other ATPases more broadly.
Time- and Temperature-Dependent Activation of Hepatitis C Virus for Low-pH-Triggered Entry
There are rate-limiting, postbinding events that are needed to render HCV competent for low-pH-triggered entry, and these events may involve interaction with a cellular coreceptor or other factors but do not require cathepsins B and L, late endosomal proteases that activate Ebola virus and reovirus for entry.
A stable full-length yellow fever virus cDNA clone and the role of conserved RNA elements in flavivirus replication.
The successful construction of a full-length infectious cDNA clone of the vaccine strain YF-17D is reported, which will aid future studies on YF replication and pathogenesis, as well as facilitate the development of YF -17D-based recombinant vaccines.
Molecular biology of flaviviruses.
The ins and outs of hepatitis C virus entry and assembly
Hepatitis C virus, a major human pathogen, produces infectious virus particles with several unique features, such as an ability to interact with serum lipoproteins, a dizzyingly complicated process