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A Radically Different Mechanism for S-Adenosylmethionine–Dependent Methyltransferases
TLDR
The mechanisms of two reactions targeting the sp2-hybridized carbons at positions 2 and 8 of adenosine 2503 in 23S ribosomal RNA, catalyzed by RlmN and Cfr, respectively are determined. Expand
Radical S-Adenosylmethionine Enzymes in Human Health and Disease.
TLDR
The biochemical characterization of these 8 radical S-adenosylmethionine enzymes is summarized and, in the context of human health, the deleterious effects that result from such genetic mutations are described. Expand
Identification of an intermediate methyl carrier in the radical S-adenosylmethionine methylthiotransferases RimO and MiaB.
TLDR
It is shown that both RimO and MiaB from Thermotoga maritima catalyze methyl transfer from SAM to an acid/base labile acceptor on the protein in the absence of their respective macromolecular substrates. Expand
Facile benzo-ring construction via palladium-catalyzed functionalization of unactivated sp3 C-H bonds under mild reaction conditions.
TLDR
A practical synthetic method for the annulation of benzo-rings by the intramolecular coupling of an aryl iodide and a methylene C-H bond is described and an additive of ortho-phenyl benzoic acid functions as a critical ligand for the catalytic process under mild condition, even at near room temperature. Expand
Transformations of the FeS Clusters of the Methylthiotransferases MiaB and RimO, Detected by Direct Electrochemistry
TLDR
The results are consistent with a mechanism whereby the auxiliary cluster is transiently methylated during substrate methylthiolation, and the redox properties of the bound cofactors within the AdoMet radical superfamily are poorly understood. Expand
Biochemistry: The ylide has landed
TLDR
The authors have determined the biosynthetic pathway of Cx-SAM, which proceeds through an unusual reactive ylide intermediate, and show that the metabolite has a role in tRNA modification in a wide range of bacteria. Expand
Stereochemical Course of the Reaction Catalyzed by RimO, a Radical SAM Methylthiotransferase.
TLDR
The stereochemical course of the RimO reaction is described, showing that RimO from Bacteroides thetaiotaomicron (Bt) catalyzes abstraction of the pro-S hydrogen atom, as evidenced by the transfer of deuterium into 5'-deoxyadenosine (5'-dAH). Expand
Trifluoroselenomethionine: A New Unnatural Amino Acid
TLDR
TFSeM shows enhanced methioninase‐induced cytotoxicity, relative to selenomethionine (SeM), toward HCT‐116 cells derived from human colon cancer, and mechanisms for this enhanced activity are computationally and experimentally examined. Expand
Emissions of Volatile Particulate Components from Turboshaft Engines Operated with JP-8 and Fischer-Tropsch Fuels
Particulate emissions from two types of helicopter turboshaft engines operated with military JP-8 and paraffinic Fischer-Tropsch (FT) fuels were characterized as an objective of the field campaignExpand
Phe496 and Leu497 are essential for receptor binding and cytotoxic action of the murine interleukin-4 receptor targeted fusion toxin DAB389-mIL-4.
TLDR
It is demonstrated that deletion of the C-terminal 15 amino acids of the fusion toxin leads to loss of cytotoxicity and the substitution of Phe496 with either Pro, Ala or Tyr results in a greater than 20-fold decrease in cytotoxic activity of the respective mutant fusion toxins. Expand
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