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Characterization of the Xenopus Rhodopsin Gene (*)
TLDR
Results locate transcriptional control elements upstream of the Xenopus rhodopsin gene and show the feasibility of embryo transfections for promoter analysis of rod-specific genes. Expand
Transgene expression in Xenopus rods
TLDR
These are the first results using transgenic Xenopus for retinal promoter analysis and the potential for the expression in rod photoreceptors of proteins with dominant phenotypes, as well as the mechanisms that regulate gene expression in a rod/cone‐specific fashion. Expand
Crystal structure of cone arrestin at 2.3A: evolution of receptor specificity.
TLDR
Functional analysis shows that cone arrestin, in sharp contrast to its rod counterpart, bound cone pigments and non-visual receptors, suggesting that it may also regulate phototransduction and/or photopigment trafficking in cone photoreceptors. Expand
Transducin Activation by the Bovine Opsin Apoprotein (*)
TLDR
The interaction of the bovine opsin apoprotein with transducin in rod outer segment membranes was investigated using a guanyl nucleotide exchange assay, and opsin showed a broad pH range for optimal activity, with no activity in buffers of pH>9, whereas metarhodopsin(II) exhibited activity at pH >9. Expand
A mechanism for exact sensory adaptation based on receptor modification.
TLDR
It is shown that judicious choice of the weights can provide both an adequate response and exact adaptation to step increases in stimuli. Expand
Rhodopsin from the fish, Astyanax: role of tyrosine 261 in the red shift.
TLDR
A naturally occurring fish rhodopsin is red shifted about 8 nm due to one critical amino acid substitution, implicated in the long wavelength absorption of the red cone pigment. Expand
Xenopus Rhodopsin Promoter
TLDR
Results suggest that rod cell-specific expression of rhodopsin is controlled by redundant elements in the proximal promoter, and none of the tested sequences were found to be necessary for rod specificity. Expand
Novel retinal photoreceptors
TLDR
It is shown that salmon vertebrate ancient opsin forms a functional photopigment in vitro, and is expressed in a subset of retinal horizontal and amacrine cells in vivo, indicating that retinal photoreception is not restricted to just rod and cone cells. Expand
Quantification of the cytoplasmic spaces of living cells with EGFP reveals arrestin-EGFP to be in disequilibrium in dark adapted rod photoreceptors
TLDR
The hypothesis is tested that enhanced green fluorescent protein (EGFP) can be used to quantify the aqueous spaces of living cells, using as a model transgenic Xenopus rods, and regions of rods having structures that exclude EGFP have highly reduced EGFP fluorescence. Expand
Rhodopsin Mutant P23H Destabilizes Rod Photoreceptor Disk Membranes
TLDR
Results show that mutant opsin disrupts OS disks, destabilizing the outer segment possibly via the formation of aggregates, contributing to photoreceptor loss. Expand
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