• Publications
  • Influence
Superoxide generation by endothelial nitric oxide synthase: the influence of cofactors.
TLDR
Results demonstrate that superoxide is generated from the oxygenase domain by dissociation of the ferrous-dioxygen complex and that occupation of the L-arginine binding site does not inhibit this process, and indicate that modulation of BH4 concentration may regulate the ratio of superoxide to nitric oxide generated by eNOS.
Measuring reactive oxygen and nitrogen species with fluorescent probes: challenges and limitations.
TLDR
A critical analysis of the challenges and limitations of the most widely used fluorescent probes for detecting and measuring reactive oxygen and nitrogen species and proposed guidelines that will help present and future researchers with regard to the optimal use of selected fluorescent probes and interpretation of results are presented.
Superoxide reacts with hydroethidine but forms a fluorescent product that is distinctly different from ethidium: potential implications in intracellular fluorescence detection of superoxide.
TLDR
It is shown that superoxide generated in several enzymatic or chemical systems oxidizes HE to a fluorescent product that is totally different from ethidium, which could have implications in intracellular detection and imaging of superoxide.
Mitochondrial metabolism and ROS generation are essential for Kras-mediated tumorigenicity
TLDR
It is reported that the major function of glucose metabolism for Kras-induced anchorage-independent growth, a hallmark of transformed cells, is to support the pentose phosphate pathway.
Redox-based regulation of signal transduction: principles, pitfalls, and promises.
TLDR
Some of the recent findings that illuminate the significance of redox signaling and exciting future perspectives are reviewed to highlight some of the current pitfalls and the approaches needed to advance this important area of biochemical and biomedical research.
Detection and characterization of the product of hydroethidine and intracellular superoxide by HPLC and limitations of fluorescence.
TLDR
Analysis of the fluorescence characteristics of ethidium (E(+)) and 2-OH-E(+) strongly suggests that the currently available fluorescence methodology is not suitable for quantitating intracellular O(2)(.-).
Mitochondrial complex III ROS regulate adipocyte differentiation.
TLDR
It is reported that primary human mesenchymal stem cells undergoing differentiation into adipocytes display an early increase in mitochondrial metabolism, biogenesis, and reactive oxygen species (ROS) generation, and this increase was dependent on mTORC1 signaling.
Teaching the basics of redox biology to medical and graduate students: Oxidants, antioxidants and disease mechanisms☆
TLDR
A brief overview of the reaction chemistry of reactive oxygen and nitrogen species, the role of various enzymes involved in the generation and detoxification of these species in disease mechanisms and drug toxicity and the protective role of dietary antioxidants is given.
Oxidation of polycyclic aromatic hydrocarbons and dibenzo[p]-dioxins by Phanerochaete chrysosporium ligninase.
TLDR
It is reported that this enzyme also catalyzes the oxidation of certain aromatic pollutants and compounds related to them, including polycyclic aromatic hydrocarbons with ionization potentials less than or equal to approximately 7.55 eV.
Synthesis and biochemical applications of a solid cyclic nitrone spin trap: a relatively superior trap for detecting superoxide anions and glutathiyl radicals.
TLDR
A novel cyclic nitrone spin trap, 5-tert-butoxycarbonyl 5-methyl-1-pyrroline N-oxide as a pure white solid has been synthesized for the first time and its favorable chemical and spectroscopic features make BMPO ideal for the detection of superoxide anions, hydroxyl and thiyl radicals in biochemical oxidation and reduction.
...
1
2
3
4
5
...