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Chemical and enzymatic analysis of covalent bonds between peptides and chromosomal DNA.
Evidence is presented indicating that the latter fraction of DNA isolated after prolonged alkaline cell lysis and phenol extraction contains phospho-triesters between hydroxy amino acid residues in peptides and internucleotide phosphates, which are most likely side products of peptide-nucleotidePhosphodiesterase-sensitive peptides released by alkali in a time and concentration-dependent reaction.
Induction of apoptosis by overexpression of the DNA-binding and DNA-PK-activating protein C1D.
C1D-induced apoptosis is discussed in relation to its potential to activate DNA-PK, which has been considered to act as an upstream activator of p53.
Schedule-dependent interaction between Doxorubicin and mTHPC-mediated photodynamic therapy in murine hepatoma in vitro and in vivo
Results indicate that Dox potentiates therapeutic efficacy of mTHPC-mediated PDT and vice versa, and the degree of potentiation is influenced by the combination schedule: administration of Dox immediately after light exposure is preferable to administration ofDox at 24 h prior to light exposure.
mTHPC‐mediated Photodynamic Treatment Up‐regulates the Cytokines VEGF and IL‐1alpha
In addition to the cytotoxic action on the A‐431 cells, mTHPC‐mediated PDT stimulated the production of VEGF and IL‐1alpha, and IL-1alpha contributed to the VEGf overexpression, establishing IL‐ 1alpha as a possible target of combined cancer treatment.
The protective effects of dihydrolipoamide and glutathione against photodynamic damage by Al‐phtalocyanine tetrasulfonate
Findings point out to the possibility of LipS2NH2/Lip(SH)2 NH2 to neutralize the side‐effects of photodynamic therapy, and to a minor but definitely protective role of GSH.
Covalent Interaction of Proteins and Nucleic Acids. Synthetic and Natural Nucleotide-Peptides
Data is reported on the occurrence, structure and functions of covalent nucleotide and NA-protein complexes, and methods of specific cleavage of phosphoamide and phosphoester bonds in nucleotide-peptides and their application in biochemistry.
High salt- and SDS-stable DNA binding protein complexes with ATPase and protein kinase activity retained in chromatin-depleted nuclei.
Cell lysis in presence of SDS and proteinase K followed by salting-out of residual polypeptides by dehydration and precipitation with saturated sodium chloride solution efficiently resolves deproteinized DNA.