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Utilization of Glyphosate as Phosphate Source: Biochemistry and Genetics of Bacterial Carbon-Phosphorus Lyase
This review deals with the radical-based mechanism employed by the carbon-phosphorus lyase of the Carbon-Phosphorus Lyase pathway, which involves reactions for activation of phosphonate, carbon- phosphorus bond cleavage, and further chemical transformation before a useful phosphate ion is generated in a series of seven or eight enzyme-catalyzed reactions. Expand
Oxygen-dependent catabolism of indole-3-acetic acid in Bradyrhizobium japonicum.
The pattern of metabolite usage by known tryptophan-auxotrophic mutants and studies of metabolites by high-performance liquid chromatography indicate that anthranilic acid is a terminal degradation product in the proposed pathway. Expand
Characterization of the Escherichia coli codBA operon encoding cytosine permease and cytosine deaminase
The nucleotide sequence of a 3.1 kb segment carrying the cytosjne deaminase gene (codA) from Esctierichia coli was determined. The sequence revealed the presence of two open reading frames, the firstExpand
Catabolism of indole-3-acetic acid and 4- and 5-chloroindole-3-acetic acid in Bradyrhizobium japonicum.
The identified products indicate a novel metabolic pathway in which IAA is metabolized via dioxindole-3-acetic acid, doxindole, isatin, and 2-aminophenyl glyoxylic acid (isatinic acid) to anthranilic acid, which is further metabolized. Expand
Location on the chromosome of Escherichia coli of genes governing purine metabolism
Genes coding for enzymes functioning in purine salvage pathways have been located on the chromosome of Escherichia coli and a deletion covering man-uidA-add was obtained. Expand
Proteome reference maps of the Lotus japonicus nodule and root
Two developmental stages representing nodules prior to nitrogen fixation (white) and mature nitrogen fixing nodules (red) were compared with roots and an indication of more pronounced PTMs in nodules than in roots was determined, suggesting a higher stress level at this developmental stage. Expand
Enzymes of ureide synthesis in pea and soybean.
The level of enzymes found in the bacteroids indicated no significant contribution of Rhizobium japonicum purine catabolism in the overall formation of ureides in the soybean nodule. Expand
Five phosphonate operon gene products as components of a multi-subunit complex of the carbon-phosphorus lyase pathway
It is concluded that phnGHIJK specify a soluble multisubunit protein complex essential for organophosphonate utilization by Escherichia coli. Expand
Microbial models and regulatory elements in the control of purine metabolism.
An analysis of 13 genes that control the biosynthesis of AMP and GMP has revealed three multigenic operons that show properties of gene contiguity, promoter sites, coordinate expression and polarity effects and a role for glutamine in the induction of GMP reductase has been revealed. Expand
Structural insights into the bacterial carbon-phosphorus lyase machinery
The crystal structure of the 240-kilodalton Escherichia coli C–P lyase core complex is determined, and it is shown that it is a two-fold symmetric hetero-octamer comprising an intertwined network of subunits with unexpected self-homologies. Expand