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Manipulating the mouse embryo: A laboratory manual
Here are recorded the tech- niques for preparing, inserting and analysing DNA sequences, for retroviral infection of mice, for production and use of EC and EK cells as vehicles for engineered sequences and for nuclear transplantation - all against a background of the basic procedures required for pro- ducing and handling the em- bryos.
Bmp4 is required for the generation of primordial germ cells in the mouse embryo.
Ch Chimera analysis indicates that it is the Bmp4 expression in the extraembryonic ectoderm that regulates the formation of allantois and primordial germ cell precursors, and the size of the founding population of PGCs.
Bone morphogenetic protein-4 is required for mesoderm formation and patterning in the mouse.
Results provide direct genetic evidence that BMP-4 is essential for several different processes in early mouse development, beginning with gastrulation and mesoderm formation.
Fibroblast growth factor 10 (FGF10) and branching morphogenesis in the embryonic mouse lung.
It is concluded that, during early lung development, localized sources of FGF10 in the mesoderm regulate endoderm proliferation and bud outgrowth.
Derivation of pluripotential embryonic stem cells from murine primordial germ cells in culture
PDX-1 is required for pancreatic outgrowth and differentiation of the rostral duodenum.
The pdx-1/beta-galactosidase fusion allele is expressed in pancreatic and duodenal cells in the absence of functional PDX-1, with expression continuing into perinatal stages with similar boundaries and expression levels, and offers additional insight into the role of p dx-1 in the determination and differentiation of the posterior foregut.
Bone morphogenetic proteins (BMPs) as regulators of dorsal forebrain development.
Evidence is provided that BMPs function during regional morphogenesis of the dorsal telencephalon by regulating specific gene expression, cell proliferation and local cell death.
Basal cells as stem cells of the mouse trachea and human airway epithelium
The pseudostratified epithelium of the mouse trachea and human airways contains a population of basal cells expressing Trp-63 and cytokeratins 5, which generate differentiated cells during postnatal growth and in the adult during both steady state and epithelial repair.
Nodal-related signals induce axial mesoderm and dorsalize mesoderm during gastrulation.
Two Xenopus nodal-related genes (Xnr-1 and Xnr-2) are identified and shown to be expressed transiently during embryogenesis, first within the vegetal region of late blastulae and later in the marginal zone during gastrulation, with enrichment in the dorsal lip.
Type 2 alveolar cells are stem cells in adult lung.
A combination of in vivo clonal lineage analysis, different injury/repair systems, and in vitro culture of purified cell populations is used to obtain new information about the contribution of AEC2s to alveolar maintenance and repair.