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Insights into the phylogeny and coding potential of microbial dark matter
TLDR
This study applies single-cell genomics to target and sequence 201 archaeal and bacterial cells from nine diverse habitats belonging to 29 major mostly uncharted branches of the tree of life and provides a systematic step towards a better understanding of biological evolution on the authors' planet.
Minimum information about a single amplified genome (MISAG) and a metagenome-assembled genome (MIMAG) of bacteria and archaea
TLDR
Two standards developed by the Genomic Standards Consortium (GSC) for reporting bacterial and archaeal genome sequences are presented, including the Minimum Information about a Single Amplified Genome (MISAG) and the Minimum information about a Metagenome-Assembled Genomes (MIMAG), including estimates of genome completeness and contamination.
Microbiology and geochemistry of great boiling and mud hot springs in the United States Great Basin
TLDR
The most abundant archaeal phylotype was closely related to “Candidatus Nitrosocaldus yellowstonii”, suggesting a role for ammonia oxidation in primary production; however, few other phylotypes could be linked with energy calculations because phylotypes were either related to chemoorganotrophs or were unrelated to known organisms.
A korarchaeal genome reveals insights into the evolution of the Archaea
TLDR
The initial characterization of a member of the Korarchaeota with the proposed name, “Candidatus Kor Archaeum cryptofilum,” which exhibits an ultrathin filamentous morphology is reported, suggesting that the organism relies on a simple mode of peptide fermentation for carbon and energy and lacks the ability to synthesize de novo purines, CoA, and several other cofactors.
Polycyclic Aromatic Hydrocarbon Degradation by a New Marine Bacterium, Neptunomonas naphthovorans gen. nov., sp. nov
TLDR
Differences in morphologic, physiologic, and genotypic differences between the new strains and the oceanospirilla justify the creation of a novel genus and species, Neptunomonas naphthovorans.
Genes for the cytoskeletal protein tubulin in the bacterial genus Prosthecobacter
TLDR
It is predicted that the Prosthecobacter tubulins are monomeric, unlike eukaryotic α and β tubulinins, which form dimers and are therefore unlikely to form microtubule-like structures.
A Comprehensive Census of Microbial Diversity in Hot Springs of Tengchong, Yunnan Province China Using 16S rRNA Gene Pyrosequencing
TLDR
A comprehensive, cultivation-independent census of microbial communities in 37 samples collected from these geothermal fields, encompassing sites ranging in temperature from 55.1 to 93.6°C, in pH from 2.5 to 9.4, and in mineralogy from silicates in Rehai to carbonates in Ruidian significantly expands the current understanding of the microbiology in Tengchong hot springs.
Isolation of Marine Polycyclic Aromatic Hydrocarbon (PAH)-Degrading Cycloclasticus Strains from the Gulf of Mexico and Comparison of Their PAH Degradation Ability with That of Puget Sound
TLDR
Three representatives were chosen for a complete phylogenetic analysis, which confirmed the close relationship of these isolates to type strain Cycloclasticus pugetii PS-1, which was isolated from Puget Sound, and supports the hypothesis that these geographically separated isolates are closely related phylogentically.
Verrucomicrobia div. nov., a new division of the Bacteria containing three new species of Prosthecobacter
TLDR
Four strains of nonmotile, prosthecate bacteria isolated in the 1970s and assigned to the genus Prosthecobacter were compared genotypically by DNA/DNA reassociation and 16S rDNA based phylogenetic analyses and complemented with phenotypic characterizations to clearly indicate each strain represents a novel species of bacteria.
Detection of 16S rDNA sequences representing the novel phylum "Nanoarchaeota": indication for a wide distribution in high temperature biotopes.
TLDR
Positive PCR amplifications from Yellowstone National Park, Uzon Caldera, and an abyssal vent system obtained sequences form a cluster with the sequence of "Nanoarchaeum equitans", indicating a wide distribution of this phylum.
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