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Industrial methods for the production of optically active intermediates.
For the industrial implementation of many transformations alternative methods are available and the advantages of the individual methods will be discussed herein and exemplified by syntheses of relevant compounds. Expand
Industrial biocatalysis today and tomorrow
Biocatalytic processes can now be carried out in organic solvents as well as aqueous environments, so that apolar organic compounds aswell as water-soluble compounds can be modified selectively and efficiently with enzymes and bioc atalytically active cells. Expand
Microbial biofilms: a concept for industrial catalysis?
A variety of biofilm reactor configurations have been explored for productive catalysis and some reactors have been operated continuously for months, which all facilitate continuous processing. Expand
Asymmetric Bioreduction of Activated C—C Bonds Using Enoate Reductases from the Old Yellow Enzyme Family
The asymmetric bioreduction of alkenes bearing an electron-withdrawing group using flavin-dependent enzymes from the 'old yellow enzyme' family at the expense of NAD(P)H yields the correspondingExpand
Identification of novel enzymes with different hydrolytic activities by metagenome expression cloning.
The results demonstrate the suitability of this direct cloning approach, which comprised a dual-orientation expression vector and a simple one-step DNA purification method, for the efficient discovery of numerous active novel clones for the rapid generation of large libraries of hitherto unknown enzyme candidates. Expand
Directed Evolution of Vibrio fischeri LuxR for Improved Response to Butanoyl-Homoserine Lactone
Variation of the four mutations within LuxR-G4E demonstrated that at least three of these changes were simultaneously required to achieve any measurable C4HSL response, suggesting that LuxR is evolutionarily pliable and not intrinsically asymmetric in its response to quorum-sensing signals with different acyl-side-chain lengths. Expand
Microbial biofilms: New catalysts for maximizing productivity of long‐term biotransformations
Biofilm physiology and dynamics are characterized during the biotransformation and limitations and advantages of this reaction concept are discussed. Expand
Synthesis of ω-hydroxy dodecanoic acid based on an engineered CYP153A fusion construct
A chimeric protein is constructed and purified where the fusion of the monooxygenase CYP153A from Marinobacter aquaeloei to the reductase domain of P450 BM3 from Bacillus megaterium ensures optimal protein expression and efficient electron coupling. Expand
Biotransformation of benzaldehyde into (R)-phenylacetylcarbinol by filamentous fungi or their extracts
Abstract. Extracts of 14 filamentous fungi were examined regarding their potential for production of (R)-phenylacetylcarbinol [(R)-PAC], which is the chiral precursor in the manufacture of theExpand