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Saccharomyces Cerevisiae Cells Secreting an Aspergillus Niger β-Galactosidase Grow on Whey Permeate
We describe the construction of a lactose-utilizing Saccharomyces cerevisiae that expresses the cDNA for a secreted, thermostable β-galactosidase (lacA) from Aspergillus niger. Yeast cells expressingExpand
  • 80
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Homologies in serine proteinases.
  • B. Hartley
  • Biology, Medicine
  • Philosophical transactions of the Royal Society…
  • 12 February 1970
Bovine pancreatic juice contains approximately equal amounts of four inactive precursors of endopeptidases (zymogens): chymotrypsinogen A, chymotrypsinogen B, trypsinogen (Keller, Cohen & NeurathExpand
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Fermentation of lactose by yeast cells secreting recombinant fungal lactase.
Strains of Saccharomyces cerevisiae transformed with a yeast multicopy expression vector carrying the cDNA for Aspergillus niger secretory beta-galactosidase under the control of ADH1 promoter andExpand
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Crystalline porcine pancreatic elastase.
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Strategy and tactics in protein chemistry.
  • B. Hartley
  • Biology, Medicine
  • The Biochemical journal
  • 1 October 1970
  • 884
  • 2
Competitive labelling, a method for determining the reactivity of individual groups in proteins. The amino groups of porcine elastase.
1. A method is described for determining the ionization constants and reactivities of individual amino groups in proteins. The principle is that in the presence of a trace amount of radioactiveExpand
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The amino acid sequence of the tyrosyl-tRNA synthetase from Bacillus stearothermophilus.
The primary structure of the tyrosyl-tRNA synthetase (TyrTS) of Bacillus stearothermophilus has been deduced from the nucleotide sequence of the cloned gene and from the amino acid sequence ofExpand
  • 81
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Histidine sequences in the active centres of some 'serine' proteinases.
1. A comparison of the diagonal ;maps' of chymotrypsin A and ;tosylphenylalanyl chloromethyl ketone'-inhibited chymotrypsin A showed that His-57 is alkylated specifically by this substrate analogue.Expand
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Ribitol dehydrogenase from Klebsiella aerogenes. Purification and subunit structure.
Ribitol dehydrogenase has been purified to homogeneity from several strains of Klebsiella aerogenes. One strain yields 3-6g of pure enzyme from 1kg of cells. The enzyme is a tetramer of fourExpand
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Purification and properties of Klebsiella aerogenes D-arabitol dehydrogenase.
An Escherichia coli K12 strain was constructed that synthesized elevated quantities of Klebsiella aerogenes D-arabitol dehydrogenase; the enzyme accounted for about 5% of the soluble protein in thisExpand
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