B. Halkier

Publications179
h-index63
Citations14,629
Highly Influential Citations775
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Biology and biochemistry of glucosinolates.
TLDR
Glucosinolates are sulfur-rich, anionic natural products that upon hydrolysis by endogenous thioglucosidases called myrosinases produce several different products that function as cancer-preventing agents, biopesticides, and flavor compounds. Expand
Biosynthesis of glucosinolates--gene discovery and beyond.
TLDR
Major breakthroughs include the ability to produce glucosinolates in Nicotiana benthamiana, the finding that specific glucos inolates play a key role in Arabidopsis innate immune response, and a better understanding of the link between primary sulfur metabolism and glucosInolate biosynthesis. Expand
Arabidopsis mutants in the C-S lyase of glucosinolate biosynthesis establish a critical role for indole-3-acetaldoxime in auxin homeostasis.
TLDR
The abolishment of glucosinolates in sur1 indicates that the SUR1 function is not redundant and thus SUR1 constitutes a single gene family, and provides important insight to the critical role IAOx plays in auxin homeostasis as a key branching point between primary and secondary metabolism, and defines a framework for further dissection of auxin biosynthesis. Expand
Glucosinolate research in the Arabidopsis era.
TLDR
There is a strong interest in the ability to regulate and optimize the levels of individual glucosinolates tissue-specifically to improve the nutritional value and pest resistance of crops. Expand
Cytochrome P450 CYP79B2 from Arabidopsis Catalyzes the Conversion of Tryptophan to Indole-3-acetaldoxime, a Precursor of Indole Glucosinolates and Indole-3-acetic Acid*
TLDR
The data show that oxime production by CYP79s is not restricted to those amino acids that are precursors for cyanogenic glucosides, which is consistent with the hypothesis that indole glucosinolates have evolved from cyanogenesis. Expand
Modulation of CYP79 Genes and Glucosinolate Profiles in Arabidopsis by Defense Signaling Pathways1
TLDR
The present data indicate that different indole glucos inolate methoxylating enzymes are induced by the jasmonate and the SA signal transduction pathways, whereas the aliphatic glucosinolates appear to be primarily genetically and not environmentally controlled. Expand
Advancing uracil-excision based cloning towards an ideal technique for cloning PCR fragments
TLDR
The largely unused uracil-excision molecular cloning technique is advanced by identifying PfuCx as a compatible proof-reading DNA polymerase and by developing an improved vector design strategy. Expand
A Systems Biology Approach Identifies a R2R3 MYB Gene Subfamily with Distinct and Overlapping Functions in Regulation of Aliphatic Glucosinolates
TLDR
Three R2R3 MYB transcription factors regulating aliphatic glucosinolate biosynthesis in Arabidopsis provide a unique system within which to study the evolution of MYB regulatory factors and their downstream targets. Expand
Biochemical Networks and Epistasis Shape the Arabidopsis thaliana Metabolome[W]
TLDR
This work conducted a replicated metabolomic analysis on 210 lines of an Arabidopsis population that was previously used for targeted metabolite quantitative trait locus (QTL) and global expression QTL analysis and found frequent epistatic interactions controlling a majority of the variation. Expand
Arabidopsis Cytochrome P450 Monooxygenase 71A13 Catalyzes the Conversion of Indole-3-Acetaldoxime in Camalexin Synthesis[W]
TLDR
It is concluded that CYP71A13 catalyzes the conversion of IAOx to IAN in camalexin synthesis and provide further support for the role of camaleXin in resistance to A. brassicicola. Expand
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