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Thymidylate Synthase Heterogeneity Assessed by Monoclonal Antibodies
Thymidylate synthase (TS; EC 22.214.171.124) catalyzes N5,10-methylenetetrahydrofolate-dependent methylation of dUMP. Purified preparations of the enzyme from regenerating rat liver, L1210 cell and… Expand
An isotopic assay of dUTPase activity based on coupling with thymidylate synthase.
A new rapid, sensitive and convenient procedure is presented allowing determination of dUTPase activity, based on measuring dUTpase-catalyzed [5-(3)H]dUMP production, to show comparable results with a previously described assay. Expand
Synthesis and interaction with thymidylate synthase of 5'-dithiophosphate and 5'-fluorothiophosphate of thymidine.
- B. Gołos, K. Misiura, M. Olesiak, A. Okruszek, W. Stec, W. Rode
- Chemistry, Medicine
- Acta biochimica Polonica
While dTMPS2 proved to be an inhibitor, competitive vs dUMP, with K(i)app = 94 microM, the 5'-fluorothiophosphate congener displayed no activity, indicating that the enzyme requires for binding the presence of a dianionic 5'-phosphate group in a nucleotide. Expand
[Chromosomal translocation and neoplastic transformation].
- B. Gołos
- Biology, Medicine
- Postepy biochemii