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Crystal structure of grape dihydroflavonol 4-reductase, a key enzyme in flavonoid biosynthesis.
The results demonstrate that position 133 cannot be solely responsible for the recognition of the B-ring hydroxylation pattern of dihydroflavonols. Expand
Annexin-A5 assembled into two-dimensional arrays promotes cell membrane repair
This work shows that annexin-A5 (AnxA5), a protein that self-assembles into two-dimensional arrays on membranes upon Ca2+ activation, promotes membrane repair and participates in a previously unrecognized step of the membrane repair process. Expand
Crystal structure and catalytic mechanism of leucoanthocyanidin reductase from Vitis vinifera.
The data support the concept that all PIP enzymes reduce a quinone methide intermediate and that the major role of the only residue that has been conserved from the short-chain dehydrogenase/reductase catalytic triad, lysine, is to promote the formation of this intermediate by catalyzing the deprotonation of a phenolic hydroxyl. Expand
Structure and epimerase activity of anthocyanidin reductase from Vitis vinifera.
The first three-dimensional structure of ANR is described at a resolution of 2.2 A and explains the inactivity of the enzyme in the presence of high salt concentrations. Expand
A single amino acid change within the R2 domain of the VvMYB5b transcription factor modulates affinity for protein partners and target promoters selectivity
The findings indicate that VvMYB5bL is still able to bind DNA but the structural consequences linked to the mutation affect the capacity of the protein to activate the transcription of some flavonoid genes by modifying the interaction with its co-partner(s). Expand
Remarkable ability of horse spleen apoferritin to demetallate hemin and to metallate protoporphyrin IX as a function of pH.
A mechanism is proposed to explain demetallation of heme, involving attack on the tetrapyrrole nitrogens of the protoporphyrin IX-Fe by protons derived from protein carboxylic acid groups and subsequent complexation of the iron by the corresponding carboxylates. Expand
Microstructure, hardness and toughness of nanostructured and conventional WC-Co composites
Abstract The microstructure and mechanical properties of nanograin-sized WC-Co composites were investigated and compared with those of conventional cermets. The dislocation density in theExpand
The epimerase activity of anthocyanidin reductase from Vitis vinifera and its regiospecific hydride transfers
It was found that deuterium was always incorporated at C2 and C4, and it was concluded that C3-epimerization should be achieved by tautomerization between the two hydride transfers and that this produces a quinone methide intermediate which serves as C4 target of the secondhydride transfer, thereby avoiding any stereospecific modification of carbon 3. Expand
A crystallographic study of haem binding to ferritin.
The present paper describes the structure of horse-spleen apo-ferritin cocrystallized with Sn-protoporphyrin IX, the iron-storage protein, which comprises 1613 non-H atoms, two Cd atoms and 170 solvent molecules. Expand
Structural evidence for the inhibition of grape dihydroflavonol 4-reductase by flavonols.
The results suggest that flavonols could be inhibitors of the activity of DFR towards dihydroflavonols. Expand