• Publications
  • Influence
Properties of purified recombinant human polyamine oxidase, PAOh1/SMO.
The results of these studies are consistent with the hypothesis that PAOh1/SMO represents a new addition to the polyamine metabolic pathway that may represent a new target for antineoplastic drug development. Expand
Induction of DNA topoisomerase II-mediated DNA cleavage by beta-lapachone and related naphthoquinones.
The structural similarity between menadione, a para-quinone, and beta-lapachone, an ortho-quinones, together with their similar activity in poisoning topoisomerase IIalpha, suggests a common mechanism of action involving chemical reactivity of these quinones. Expand
Beta-lapachone-mediated apoptosis in human promyelocytic leukemia (HL-60) and human prostate cancer cells: a p53-independent response.
beta-Lapachone and certain of its derivatives directly bind and inhibit topoisomerase I (Topo I) DNA unwinding activity and form DNA-Topo I complexes, which are not resolvable by SDS-K+ assays. WeExpand
Reaction of beta-lapachone and related naphthoquinones with 2-mercaptoethanol: a biomimetic model of topoisomerase II poisoning by quinones.
The results support the hypothesis that the cytotoxic effect of the naphthoquinones derive, at least in part, from their alkylation of exposed thiol residues on the topoisomerase II-DNA complex. Expand
Pyrrolooxygenases: isolation, properties, and products formed.
A new group of enzymes was isolated from wheat germ and rat liver which oxidized the pyrrole ring of indoles affording o -formamidophenacyl derivatives, and were named pyrrolooxygenases, which had an absolute requirement for oxygen and an exogenous reducing agent. Expand
Pyrroles from azaindoles. A synthesis of porphobilinogen and related pyrroles.
Purification and properties of porphobilinogen deaminase from wheat germ.
Porphobilinogen deaminase from wheat germ was purified about 1000-fold and a dithionite-activated protein factor was found to consume PBG in a special way and to interact with the PBG-deaminase by inhibiting its activity. Expand
Carbon-13 nuclear magnetic resonance analysis of [1-13C]glucose metabolism in Trypanosoma cruzi. Evidence of the presence of two alanine pools and of two CO2 fixation reactions.
The non-invasive technique of 13C-nuclear magnetic resonance was applied to study glucose metabolism in vivo in Trypanosoma cruzi, the causative agent of American trypanosomiasis, and revealed the presence in T. cruzi of a reductive pathway of CO2 which is independent of the CO2 fixation reaction. Expand
Conformationally restricted analogues of 1N,14N-bisethylhomospermine (BE-4-4-4): synthesis and growth inhibitory effects on human prostate cancer cells.
Four new tetramines, the two cyclopropyl isomers, the trans-cyclobutyl isomer, and the (5Z)-tetraazaeicosene, were more cytotoxic than their saturated counterpart (BE-4-4 -4) but could not be correlated either with their cellular uptake or with their ability to deplete intracellular polyamine pools. Expand