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Eight histidine residues are catalytically essential in a membrane-associated iron enzyme, stearoyl-CoA desaturase, and are conserved in alkane hydroxylase and xylene monooxygenase.
Observations suggest that the membrane fatty acid desaturases and hydrocarbon hydroxylases have a related protein fold, possibly arising from a common ancestral origin. Expand
Haloalkene oxidation by the soluble methane monooxygenase from Methylosinus trichosporium OB3b: mechanistic and environmental implications.
The occurrence of atomic migration products shows that an intermediate in the substrate to product conversion carries significant cationic character in a soluble, three-protein component methane monooxygenase species, which could be generated through interaction with a highly electron-deficient activated oxygen in the active site. Expand
Methane monooxygenase from Methylosinus trichosporium OB3b. Purification and properties of a three-component system with high specific activity from a type II methanotroph.
The data presented here provide the first evidence based on catalysis that the site of the monooxygenation reaction is located on the hydroxylase, and implies a new mechanism for the generation of highly reactive oxygen capable of insertion into unactivated carbon-hydrogen bonds. Expand
Maltose-neopentyl glycol (MNG) amphiphiles for solubilization, stabilization and crystallization of membrane proteins
A class of amphiphiles, each built around a central quaternary carbon atom derived from neopentyl glycol, with hydrophilic groups derived from maltose, showing favorable behavior relative to conventional detergents, as manifested in multiple membrane protein systems, leading to enhanced structural stability and successful crystallization. Expand
Cloning and Sequence Analysis of Two PseudomonasFlavoprotein Xenobiotic Reductases
ABSTRACT The genes encoding flavin mononucleotide-containing oxidoreductases, designated xenobiotic reductases, fromPseudomonas putida II-B and P. fluorescens I-C that removed nitrite fromExpand
A combined approach to improving large-scale production of tobacco etch virus protease.
A quantitative, high-throughput assay for TEV protease activity in cell lysates is used to evaluate the efficacy of combining several previous modifications with new expression hosts and induction methods to test whether further improvements are possible. Expand
Methane monooxygenase from Methylosinus trichosporium OB3b.
X-ray Structure of a Mammalian Stearoyl-CoA Desaturase
The structure of mouse SCD1 bound to stearoyl-CoA shows a novel fold comprising four transmembrane helices capped by a cytosolic domain, and a plausible pathway for lateral substrate access and product egress, and illustrates a possible route for electron transfer from cytochrome b5 to the di-iron centre. Expand
Structure and mechanism of mouse cysteine dioxygenase.
Metal analysis showed that the recombinant enzyme contained a mixture of iron, nickel, and zinc, with increased iron content associated with increased catalytic activity. Expand
Transformation of 2,4,6-Trinitrotoluene by Purified Xenobiotic Reductase B from Pseudomonas fluorescensI-C
Reactions of chemically and enzymatically synthesized and high-pressure liquid chromatography-purified TNT metabolites showed that both a hydroxylamino-dinitrotoluene isomer and a tautomer of the protonated dihydride-Meisenheimer complex of TNT were required precursors for the dimerization and nitrite release reactions. Expand