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Albumin standards and the measurement of serum albumin with bromcresol green.
Abstract A rapid and reliable method for measuring serum albumin employing bromcresol green is described. The addition of albumin to a solution of bromcresol green in a 0.075 M succinate buffer pHExpand
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  • 55
Serum and urine albumin: a progress report on their measurement and clinical significance.
  • B. Doumas, T. Peters
  • Medicine
  • Clinica chimica acta; international journal of…
  • 3 February 1997
For about 25 years, bromcresol green and bromcresol purple have been the basis for most of the measurements of serum albumin in the US and perhaps in the world. The longevity of the methods is due toExpand
  • 167
  • 8
Standards for total serum protein assays--a collaborative study.
  • B. Doumas
  • Chemistry, Medicine
  • Clinical chemistry
  • 1 July 1975
We have studied the standardization of total serum protein assay with the biuret reaction. Standard solutions were prepared from lyophilized preparations of human serum albumin and bovine serumExpand
  • 301
  • 6
A candidate reference method for determination of total protein in serum. I. Development and validation.
We developed a candidate Reference Method for measuring total serum protein by use of the biuret reaction. The method involves a previously described biuret reagent (Clin. Chem. 21: 1159, 1975) andExpand
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  • 5
  • PDF
Candidate reference method for determination of total bilirubin in serum: development and validation.
This candidate Reference Method for measuring total bilirubin in serum is based on the Jendrassik-Gróf principle (Clin Chem 29: 297-301, 1983). Standard Reference Material no. 916 bilirubin (NationalExpand
  • 174
  • 5
The measurement of bilirubin fractions in serum.
  • B. Doumas, T. Wu
  • Chemistry, Medicine
  • Critical reviews in clinical laboratory sciences
  • 1991
Bilirubin fractions are measured by (1) the direct diazo reaction, (2) high-performance liquid chromatography (HPLC), (3) direct spectrophotometry, and (4) enzymatic methods. HPLC, which effectsExpand
  • 70
  • 5
Standardization in bilirubin assays: evaluation of selected methods and stability of bilirubin solutions.
Because of the inaccuracy of bilirubin (BIL) determinations, we have investigated some sources of error associated with the most commonly used methods. Inadequate standardization appears to be theExpand
  • 263
  • 4
Differences between values for plasma and serum in tests performed in the Ektachem 700 XR Analyzer, and evaluation of "plasma separator tubes (PST)".
We measured 25 analytes in plasma and serum from the same blood specimen, using the Kodak Ektachem 700 XR Analyzer. For 22 of the analytes, values for plasma were practically the same as those forExpand
  • 54
  • 4
  • PDF
A candidate reference method for determination of total protein in serum. II. Test for transferability.
The transferability of the candidate Reference Method for total serum protein was tested in eight laboratories in the United States and Europe. National Bureau of Standards SRM 927 (bovine serumExpand
  • 82
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Lactate dehydrogenase activities in serum and plasma.
We investigated lactate dehydrogenase activity in serum and plasma because of the conflicting data found in the literature. We assayed serum, platelet-rich, and platelet-poor plasma by twoExpand
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