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Natural antisense (rTSalpha) RNA induces site-specific cleavage of thymidylate synthase mRNA.
Results demonstrated that the antisense region of rTSalpha RNA is necessary and sufficient for this down-regulation of TS mRNA, and indicated that TS mRNA is cleaved in vivo in a site-specific manner. Expand
Human thymidylate synthetase derived from blast cells of patients with acture myelocytic leukemia. Purification and chracterization.
Initial velocity and product inhibition studies reveal the enzymic mechanism to be ordered sequential. Expand
Lack of dihydrofolate reductase in human tumor and leukemia cells in vivo.
Dihydrofolate reductase (DHFR), the main target for methotrexate and other antifolate compounds was found to be present in 100-200 times higher concentration in human cell lines grown in vitro thanExpand
5-Fluorouracil augmentation of dihydrofolate reductase gene transcripts containing intervening sequences in methotrexate-resistant KB cells.
Results suggest that 5-fluorouracil incorporation into RNA may inhibit the conversion of precursor mRNA to mature mRNA, and suggest that dihydrofolate reductase precursor mRNA specificity is under threat. Expand
Effects of 5-fluorouracil on dihydrofolate reductase and dihydrofolate reductase mRNA from methotrexate-resistant KB cells.
The hypothesis of translational miscoding during protein synthesis as a major mechanism of 5-fluorouracil-mediated cytotoxicity-methotrexate antagonism is supported and a new mechanism of 3H-reinforced dihydrofolate reductase gene-amplified KB cells is suggested. Expand
Antisense agents in cancer research and therapeutics.
  • B. Dolnick
  • Chemistry, Medicine
  • Cancer investigation
  • 1991
Multiple messenger RNAs for dihydrofolate reductase.
It is concluded that within the dihydrofolate reductase gene-amplified cell line L5178Y C 3 there are multiple messenger RNAs coding for dihydofolate reductionase and that this multiple translatable mRNA phenomenon is not the result of gene amplification. Expand
Comparative genomic analysis reveals a novel mitochondrial isoform of human rTS protein and unusual phylogenetic distribution of the rTS gene
The presence of two rTS isoforms in mitochondria suggests that the rTS signaling pathway may be active within mitochondria and is an example of identifying novel protein isoforms and for improving gene annotation through comparative genomic analysis. Expand
Correlation of dihydrofolate reductase elevation with gene amplification in a homogeneously staining chromosomal region in L5178Y cells
Molecular hybridization of a purified [3H]DNA probe complimentary to DHFR specific mRNA with cellular DNA and RNA indicates that DHFR coding sequences are elevated several hundred fold in both nucleic acid species in the mutant cell line. Expand